Data from: Gene flow in the anemone Anthopleura elegantissima limits signatures of local adaptation across an extensive geographic range
Cite this dataset
Cornwell, Brendan (2020). Data from: Gene flow in the anemone Anthopleura elegantissima limits signatures of local adaptation across an extensive geographic range [Dataset]. Dryad. https://doi.org/10.25338/B8K33X
Abstract
Methods
Individuals were collected from Washington state to Baja California. After DNA extraction, RAD-seq libraries were produced using sbfI and random shearing to achieve the desired insert size. Individuals were sequenced on the Illumina 2500 and 4000 platforms. Subsequent SNP calls were made using the STACKS software pipeline after aligning reads to a draft A. sola genome assembly. Finally, loci were screened for contamination by BLASTing all loci to a database of bacteria and dinoflagellate genomes and were excluded if they had positive BLAST match e-values of 1 or less. All remaining loci were included in subsequent analyses and are included in the dataset uploaded here. Raw reads and the draft genome assembly are available on NCBI Bioproject PRJNA632874.
Usage notes
Individual metadata including latitude/longitude where they were collected are in an associated metafile.
Funding
National Science Foundation, Award: OCE-1459815
University of California, Riverside
UC Davis Natural Reserve System
UC Davis Center for Population Biology