Exposure to teflubenzuron reduces drought tolerance of collembolans
Data files
Jun 03, 2024 version files 41.45 KB
Abstract
This is the raw data from an experiment investigating the effects of sequential exposure to a pesticide (teflubenzuron) and three abiotic stressors (heat, cold, and drought) on soil arthropods. We exposed adult collembolans (Folsomia candida) to teflubenzuron through food for two weeks. Then survivors were immediately divided into three groups for acute heat, cold, and drought exposure. After acute exposure to these natural stressors, the collembolans were moved to optimal conditions for a one-week recovery period during which their survival, time to regain reproduction, and egg production were examined.
README: Data for sequential exposure of a pesticide and abiotic stressors
https://doi.org/10.5061/dryad.1g1jwsv47
This is the dataset for the sequential exposure experiment. First, the test animals were exposed to teflubenzuron through food for 14 days. Survivors continued to be exposed to four different levels of heat stress (33.5 ℃), cold stress (-5 ℃), or drought stress.
Description of the data and file structure
There are four spreadsheets in the dataset, namely "Heat", "Cold", "Drought", and "survival tfb". The description of each spreadsheet is shown as follows.
Sheet Heat contains the survival and number of eggs during recovery and the time to regain reproduction for the heat stress (33.5 ℃) treatment. Column "tfb" stores the test concentrations of teflubenzuron, which are 0, 0.043, 0.094, 0.21, 0.48, and 1.1 mg teflubenzuron/kg dry yeast. Column heat contains the exposure time at 33.5 ℃, which are 0, 0.5, 1, and 2 h. 1 is the shortest exposure time, 30 mins and 3 is the longest exposure time, 2 h. Column rep stores the number of replicate. There are five replicates. Column total contains the total number of the survivors to begin with. Column alive_no_day7 is the number of alive animals at the end of recovery period. Column delaying_day is the number of delayed day in the unit day. Column egg_no is the total number of eggs during recover period per replicate. Column egg_div_indi is the number of eggs during recover period per total number of animals started with.
Sheet Cold contains the survival and number of eggs during recover period and the time to regain reproduction for the cold stress (-5 ℃) treatment. Column cold contains the exposure time at -5 ℃, which are 0, 0.5, 1, and 2 h. The rest of column headers are same as those in Sheet Heat.
Sheet Drought contains the survival and number of eggs druing recover period and the time to regain reproduction for the drought treatment. Column drought contains the four relative humidities, they are 0.31, 1.55, 2.53, and 3.11 -MPa. The last column note lists the reasons why I took out that particular replicate from the data analysis. All the rest column headers are same as previously described.
Sheet survival tfb contains the survival of animals after 14 days exposure to teflubenzuron. No.Petri dish is the id I gave to each Petri dish for the sake of randomnize the dishes. day0 is the number of animals that I start with for each dish. It wasaround 60 individuals. day14 is the number of alive animals at the end day of exposure. tfb is short for teflubenuzuron and it refers to the dose of teflubenzuron in the unit of mg/kg dry yeast. Survival (%) 1 is the survival rate which is calculated by number in day 14 divided by day 0. Some of the values exceed 100% due to small difference between the counting. For those exceed 100%, I corrected it to 100% as see in Survival (%) final.
The empty cells are called null. meaning no available data for those treatments.
Methods
This study utilizes original data collected from the experiment comprehensively described in a related paper. Details regarding the processing of the raw data are also provided in the same paper.