140 Male migrant workers aged 20–51 years of either Bangladeshi or Indian ethnicity from a single dormitory in Singapore volunteered to participate in the study. In total, 133 blood samples were taken at the start of the study and were used to assess vitamin B12, hemoglobin, ferritin, folate, and zinc status; a sub-sample underwent for homocysteine testing. Anthropometric measurements and vital signs, including height, weight, and blood pressure were recorded before and after the intervention.

A Clinical Research Organisation (CRO) has collected the data during the nutrition intervention study. The CRO has entered the dataset into a database (Excel file). Protocol violations and deviations, study product consumption and study completion data were evaluated during the data review to define the per protocol dataset before database lock. An independent statistician performed the statistics of the data. For all analyses, statistical significance was defined as a p-value <0.05. SPSS version 26 software (IBM Corp., Released 2019, Armonk, NY, USA) was used for all analyses. An Intent-to-treat analysis was considered for the data analysis. Analysis of outcomes was done with the original continuous measurement values. To minimize the inter-individual component covariance, the difference between baseline and endline measurements for each individual survey subject was calculated. P-values were calculated using a one-sample t-test comparing the average difference to zero. A two-sample or independent t-test was used to determine if mean values of subgroups were statistically significantly different.

Excel is needed to open the data files, and SPSS or Word is needed to open the statistical outcomes.