Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex
Data files
Dec 26, 2023 version files 102.76 KB
Abstract
Social isolation causes profound changes in social behaviour in a variety of species. However, the genetic and molecular mechanisms modulating behavioural responses to social isolation and social recovery remain to be elucidated. Here, we quantified the behavioural response of vinegar flies to social isolation using two distinct protocols (social space preference and spontaneous tendencies to form groups). We found that social isolation increased social space and reduced sociability. These effects of social isolation were reversible and could be reduced after 3 days of group housing. Flies with a loss of function of neuroligin3 (ortholog of autism-related neuroligin genes) with known increased social space in a socially enriched environment, were still able to recover from social isolation. We also show that dopamine is needed for a response to social isolation and recovery in males but not in females. Furthermore, only in males, dopamine levels are reduced after isolation and are not recovered after group housing. Finally, in socially enriched flies mutant for neuroligin3, dopamine levels are reduced in males, but not in females. We propose a model to explain how dopamine and neuroligin3 are involved in the behavioural response to social isolation and its recovery in a dynamic and sex-specific manner.
README
This README file was generated on December 21, 2023 by Ryley Yost.
GENERAL INFORMATION
Title of Dataset: Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex.
Author Information
A. Principal Investigator Contact Information (Corresponding Author)
Name: Anne Simon
Institution: Western University
Address: London, ON, Canada
Email: asimon28@uwo.caB. Associate or Co-investigator Contact Information
Name: Ryley Yost
Institution: Western University
Address: London, ON, Canada
Email: ryost@uwo.caDate of data collection (single date, range, approximate date): 2017-2023
Geographic location of data collection: London, ON, Canada & Hamilton, ON, Canada
Information about funding sources that supported the collection of the data: NSERC DG 05054-2022, NSERC DG 05420-2019.
SHARING/ACCESS INFORMATION
Licenses/restrictions placed on the data: None
Links to publications that cite or use the data:
Yost, Ryley T, Scott, Andrew M, Kurbaj, Judy M, Walshe-Roussel, Brendan, Dukas, Reuven, Simon, Anne F. (Forthcoming 2023-2024). Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex.
Links to other publicly accessible locations of the data: None
Links/relationships to ancillary data sets: None
Was data derived from another source? No
A. If yes, list source(s): NARecommended citation for this dataset:
Yost, Ryley T et al. (Forthcoming 2023-2024). Data from: Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex [Dataset]. Dryad. https://doi.org/10.5061/dryad.59zw3r2fs.
DATA & FILE OVERVIEW
- File List:
A) Figure_1.xls
B) Figure_2.xls
C) Figure_3.xls
D) Figure_4.xls
E) Figure_5.xls
F) Supplemental_Figure_3.xls
Relationship between files, if important: None
Additional related data collected that was not included in the current data package: None
Are there multiple versions of the dataset? No
A. If yes, name of file(s) that was updated: NA
i. Why was the file updated? NA
ii. When was the file updated? NA
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DATA-SPECIFIC INFORMATION FOR: Figure_1.xls
Specific graphs (Figure 1A to E) are seperated by tabs in the file.
Number of variables: 3 for each figure, 5 total
Number of cases/rows: 33-54 depending on the figure
Variable List:
* Days_of_isolation: # of days of isolation
* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)
* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)
* sex: sex of the flies (male or female)
* aggregation index: sociability score or aggregation index for all treatments in the sociability assay (variance/mean # of flies in chamber)
Missing data codes: None
Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery
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DATA-SPECIFIC INFORMATION FOR: Figure_2.xls
Specific graphs (Figure 2A to D) are seperated by tabs in the file.
Number of variables: 3 for Figure 2A & B, 2 for Figure 2C & D, 4 total
Number of cases/rows: 45-56 depending on the figure
Variable List:
* mating_status: mating status of flies used in behavioural experiments (either mated or virgin)
* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)
* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)
* rearing_density: single housed (0) or # of flies group housed (2,6,16 & random uncontrolled number) prior to the social space assay (# of flies)
Missing data codes: None
Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery
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DATA-SPECIFIC INFORMATION FOR: Figure_3.xls
Specific graphs (Figure 3A to D) are seperated by tabs in the file.
Number of variables: 3 for each figure, 3 total
Number of cases/rows: 36-41 depending on the figure
Variable List:
* genotype: genotype of flies used (either Cs [our control] or the nlg3Def1 mutant flies)
* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)
* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)
Missing data codes: None
Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery
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DATA-SPECIFIC INFORMATION FOR: Figure_4.xls
Specific graphs (Figure 4A to E) are seperated by tabs in the file.
Number of variables: 3 for Figure 4A to D, 6 total
Number of cases/rows: 35-49 depending on the figure
Variable List:
* genotype: genotype of flies used (either Cs [our control] or the nlg3Def1 mutant flies)
* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)
* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)
* sex: sex of the flies (male or female)
* age: age of the flies during testing for dopamine levels (in days)
* dopamine_levels: dopamine levels in fly heads (picograms/head)
Missing data codes: None
Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery
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DATA-SPECIFIC INFORMATION FOR: Figure_5.xls
Specific graphs (Figure 5A & B) are seperated by tabs in the file.
Number of variables: 3 for Figure 5A, 4 for Figure 5B
Number of cases/rows: 12 for Figure 5A, 11 for Figure 5B
Variable List:
* genotype: genotype of flies used (either Cs [our control] or the nlg3Def1 mutant flies)
* sex: sex of the flies (male or female)
* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)
* age: age of the flies during testing for dopamine levels (in days)
* dopamine_levels: dopamine levels in fly heads (picograms/head)
Missing data codes: None
Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery
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DATA-SPECIFIC INFORMATION FOR: Supplemental_Figure_3.xls
Specific graphs (Supplemental Figure 3A & B) are seperated by tabs in the file.
Number of variables: 3
Number of cases/rows: 29-33
Variable List:
* genotype: genotype of flies used (either Cs [our control] or the nlg3Def1 mutant flies)
* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)
* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)
Missing data codes: None
Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery
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Methods
Please see Methods in Yost et al., 2024.