Here, we present a microscopy method to image ¹⁸F-fluorodeoxyglucose and other radiotracers in patient-derived tumor organoids with spatial resolution up to 100-fold better than that of clinical positron emission tomography (PET). When combined with brightfield imaging, this metabolic imaging approach functionally mirrors clinical PET/CT scans and provides a quantitative readout of cell glycolysis other metabolic processes. 

The dataset was collected in a custom-built radioluminescence microscope setup. The images are collected by an EMCCD (Hamamatsu). The images are raw and unprocessed. 

Each image file is accompanied by a metafile that documents all the settings used for EMCCD acquisition. The name of the folders describes the experiment or specific modality used for imaging. The readme file summarizes the abbreviations used in the folder names.