Skip to main content
Dryad

Data from: Cell type- and transcription-independent spatial proximity between enhancers and promoters

Data files

Aug 23, 2024 version files 283.23 MB

Abstract

Cell type-specific enhancers are critically important for lineage specification. The mechanisms that determine cell type-specificity of enhancer activity, however, are not fully understood. Most current models for how enhancers function invoke physical proximity between enhancer elements and their target genes. Here, we use an imaging-based approach to examine the spatial relationship of cell type-specific enhancers and their target genes with single cell resolution. Using high-throughput microscopy, we measure the spatial distance from target promoters to their cell type-specific active and inactive enhancers in individual pancreatic cells derived from distinct lineages. These images were processed to identify nuclei and the loci within them. The shared dataset is composed of spot positions for promoter and enhancer probes in individual cells. It is comprised of 14 separate files, each corresponding to an individual plate imaged on a different day. Column headers and contents are consistent between individual files.