This work deals with evaluating the performance of silver nanoparticles (AgNPs) synthesized from sodium caseinate as green biological active agent, in comparison with famous used carboxymethyl cellulose and other carbohydrates (oxidized nanocellulose fibers (OC) and starch (St)). The TGA, FT-IR, TEM as well as its anti-biological behaviour toward gram negative bacteria, gram positive bacteria and Candida albicans .are examined. With regard to its anti-tumour activity, the evaluation is studied via many cancer cell lines against RPE1 (normal retina cell line)]. The data obtained reveal that, the SC-Ag(I) and CMC-Ag(I) complexes are formed in six and five-membered ring geometrical structures; respectively, as nanoparticles; while linear chelation structure is formed in case of OC-Ag (I) and St-Ag(I) complexes. The SC-AgNPs, provides greatest inhibition zone, especially +ve bacteria and fungus. It is recommended as promising and safety anti-cancer treating agents, especially towards HCT116 and PC3 (IC50 25.8 µg/ml & 45.1 µg/mL and SI 2.8->3.9).

Evidence of biopolymer-Ag complexes formation. - Estimation of reacted silver ions was determined by UV-visible Analysis according to Ref [29]. - FTIR Spectra: Infrared spectra were recorded with a Jasco FT/IR, Nicolet, and Model 670. The samples were mixed with KBr and pressed as tablets. The technique of Nelson and O’Connor [30] was used to calculate the crystallinity index (Cr.I). The mean strength of hydrogen bonds (MHBS) was calculated according to Levdik et al.[31]. - Thermogravimetric analysis (TGA): The non-isothermal TGA of the synthesized Ag- complexes in nanoparticle form, from different biopo;ymers was carried out by using Instrument SDT Q600 V20 Build 20 module (made at US), under nitrogen atmosphere at a heating rate of 10 oC/min. and temperature range from ~ 30 oC to ~1000 oC. The calculations of TGA-measurements were carried out according to References [32, 33]

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