We studied patterns of genetic differentiation and barriers to gene flow within and between morphs of T. urticae in order to understand the factors that influence the spread of resistance mutations across its populations. We derived multiple iso-female lines from Tetranychus populations collected from agricultural crops. We generated genomic and morphological data, characterized their bacterial communities, and performed controlled crosses.

The datasets were collected in controlled laboratory experiments involving reciprocal crosses and morphological measurements using different populations of Tetranychus mites.