Data from: Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model
He, Gang et al. (2010), Data from: Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model, Dryad, Dataset, https://doi.org/10.5061/dryad.8164
Molecular studies using trace DNA, such as from museum specimens, ancient or forensic samples and samples obtained noninvasively, often have a common problem of low quality of DNA templates. Amplification errors, such as allelic dropout and false allele, may arise during polymerase chain reaction (PCR) using such samples. A mathematical model which treats homozygotes and heterozygotes discriminately has been developed to measure sample quality and compute the confidence level of using multiple-tube approaches. We use plucked hair samples collected from 26 individual Sichuan snub-nosed monkeys (Rhinopithecus roxellana) to test the model. In this case, a confidence level of 99% can be achieved by three positive PCRs. If the sample quality is very poor and requires many PCR replicates, an alternative multiple-step genotyping method is recommended. This model enables researchers to optimize experimental protocols through pilot studies and obtain reliable genetic information using noninvasive sampling method.
People's Republic of China
Zhouzhi National Nature Reserve