Native plant diversity generates microbial legacies that either promote or suppress non-natives, depending on drought history
Data files
May 13, 2024 version files 15.60 MB
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Appendix_S1_Information_for_native_non-native.xlsx
11.33 KB
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Data01_water_content_condition_phase.csv
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Data02_native_biomass_condition_phase.csv
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Data03_all_fungi_unrarefied_condition_phase.csv
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Data04_all_fungi_rarefied_condition_phase.csv
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Data05_pathogen_unrarefied_condition_phase.csv
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Data06_pathogen_rarefied_condition_phase.csv
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Data07_AMF_unrarefied_condition_phase.csv
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Data08_AMF_rarefied_condition_phase.csv
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Data09_water_content_feedback_phase.csv
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Data10_invasive_biomass_feedback_phase.csv
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Data11_microbe_condition_phase_invasive_biomass_feedback_phase.csv
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README_lett.docx
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README.md
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Jul 31, 2024 version files 5.65 MB
Abstract
Diverse native plant communities resist non-native plants more than species-poor communities, in part through resource competition. The role of soil biota in diversity-invasibility relationships is poorly understood, although non-native plants interact with soil biota during invasions. We tested the responses of non-native plants to soil biota generated by different native plant diversities. We applied well-watered and drought treatments in both conditioning and response phases to explore the effects of “historical” and “contemporary” environmental stresses. When generated in well-watered soils, the microbial legacies from higher native diversity inhibited non-native growth in well-watered conditions. In contrast, when generated in drought-treated soils, the microbial legacies from higher native diversity facilitated non-native growth in well-watered conditions. Contemporary drought eliminated microbial legacy effects on non-native growth. We provide a new understanding of mechanisms behind diversity-invasibility relationships and demonstrate that temporal variation in environmental stress shapes relationships among native plant diversity, soil biota and non-native plants.
README: Native plant diversity generates microbial legacies that either promote or suppress non-natives, depending on drought history
Metadata_ the latest version_07-31-2024
Zhibin Tao1,2, Kaoping Zhang1,2, Ragan M. Callaway3, Evan Siemann4, Yanjie Liu5, Wei Huang1,2*
1 Key Laboratory of Lake and Watershed Science for Water Security, Chinese Academy of Sciences, Wuhan 430074, China
2 Hubei Key Laboratory of Wetland Evolution & Ecological Restoration, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
3 Division of Biological Sciences, University of Montana, Missoula 59812, USA
4 Department of Biosciences, Rice University, Houston 77005, USA
5 Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
Appendix_S1_Information_for_native_species
Information for native species and species assemblage used in the conditioning phase (phase I).
Appendix_S2_Information_for_non-native_species
Information for non-native species used in the responding phase (phase II).
Appendix_S3_R_codes_Tao_et_al.
R codes for all the data analysis and all figures.
Data01_soil_pathogen_rarefied
Data02_soil_AMF_rarefied
Data06_soil_all_fungi_rarefied
| log_NSR | Log-transformed $$Native species richness (1, 2, 4 and 8)]. |
|---|---|
| native_assemblage | A1-A40, 10 assemblages were established for each native species richness in the conditioning phase (phase I), see Appendix_S1 for details. |
| WPI | Watering treatments in conditioning phase (phase I): watered (~30% volumetric water content), drought (~10% volumetric water content). |
| 77d2c3234e4c95e455dc3bc44b5ea62f | ASV_ID, number of reads for ASV |
| …… | …… |
Data03_soil_microbe_and_nonnative_biomass
| log_NSR | Log-transformed $$Native species richness treatment (1, 2, 4 and 8)]. |
|---|---|
| native_assemblage | A1-A40, 10 assemblages were established for each native species richness in the conditioning phase (phase I), see Appendix_S1 for details. |
| WPI | Watering treatments in conditioning phase (phase I): watered (~30% volumetric water content), drought (~10% volumetric water content). |
| replicate | Two replicates (ab, cd). |
| fungi_richness | Richness of all fungi at the end of the conditioning phase (phase I). |
| AMF_richness | Richness of AMF at the end of the conditioning phase (phase I). |
| pathogen_richness | Richness of soil pathogens at the end of the conditioning phase (phase I). |
| average_nonnative_biomass (g) | The average biomass of 10 non-native species in each soil inocula. |
Data04_nonnative_biomass
| log_NSR | Log-transformed $$Native species richness treatment (1, 2, 4 and 8)]. |
|---|---|
| native_assemblage | A1-A40, 10 assemblages were established for each native species richness in the conditioning phase (phase I), see Appendix_S1 for details. |
| WPI | Watering treatments in conditioning phase (phase I): watered (~30% volumetric water content), drought (~10% volumetric water content). |
| WPII | Watering treatment in responding phase (phase II): watered (~30% volumetric water content), drought (~10% volumetric water content). |
| WPI_WPII | watering treatment combination $$Phase I (watered vs. drought) & Phase II (watered vs. drought), watered&watered, drought&watered, watered&drought, drought&drought] |
| replicate | Two replicates (ab, cd) |
| soil_inocula | Soil inoculum generated in conditioning phase (phase I), Native_species_richness&native_assemblage&WPI&replicate, for each treatment, see above for detail. |
| nonnative_species | 10 non-native species were used in the responding phase, see Appendix S1 for details. |
| biomass (g) | Biomass for non-native species at the end of the responding phase (phase II). We harvested aboveground and belowground biomass for each plant, dried them at 80 °C for 48 hours, and weighed them. |
Data05_native_biomass
| log_NSR | Log-transformed $$Native species richness treatment (1, 2, 4 and 8)]. |
|---|---|
| native_assemblage | A1-A40, 10 assemblages were established for each native species richness in the conditioning phase (phase I), see Appendix_S1 for details. |
| water_treatment | watered (~30% volumetric water content), drought (~10% volumetric water content) |
| block | Block number (a, b, c and d). |
| biomass (g) | Aboveground biomass of native assemblage at the end of the conditioning phase (phase I). We harvested aboveground parts, dried them at 80 °C for 48 hours, and weighed them. |
Data07_soil_microbe_composition
| fungi_group | Two groups (AMF and pathogen) |
|---|---|
| taxon | Taxon name at family level |
| ratio | Relative number of reads at family level |
Changelog
| Old version [05-13-24] | Latest version [07-31-24] |
|---|---|
| Data01_water_content_condition_phase | |
| Data02_native_biomass_condition_phase | Data05_native_biomass |
| Data03_all_fungi_unrarefied_condition_phase | |
| Data04_all_fungi_rarefied_condition_phase | Data06_soil_all_fungi_rarefied |
| Data05_pathogen_unrarefied_condition_phase | |
| Data06_pathogen_rarefied_condition_phase | Data01_soil_pathogen_rarefied |
| Data07_AMF_unrarefied_condition_phase | |
| Data08_AMF_rarefied_condition_phase | Data02_soil_AMF_rarefied |
| Data09_water_content_feedback_phase | |
| Data10_invasive_biomass_feedback_phase | Data04_nonnative_biomass |
| Data11_microbe_condition_phase_invasive_biomass_feedback_phase | Data03_soil_microbe_and_nonnative_biomass |
| Data07_soil_microbe_composition |
Note: According to the journal editor’s comments, we removed those datasets (in the old version) that were not used in data analyses, these datasets included Data01_water_content_condition_phase, Data03_all_fungi_unrarefied_condition_phase, Data05_pathogen_unrarefied_condition_phase, Data07_AMF_unrarefied_condition_phase and Data09_water_content_feedback_phase.
Moreover, we added a dataset (Data07_soil_microbe_composition for Fig. S3) in the new version.