https://doi.org/10.5061/dryad.cz8w9gjbs

We have uploaded the raw dynamic light scattering (DLS) size distribution data for tetraphenylethylene (TPE)-functionalized monotopic and tetratopic quaternary ammonium derivatives G1, G2, and their binding with host molecule tribenzotriquinacene-based hexacarboxylic acid ammonium salt H to form  H-G1 and H-G2 aggregates, the raw zeta potential data for H-G1 and H-G2 (file name: DLS_data.xlsx), and the raw fluorescence data for Figure 4 (file name: Figure_4_Fluorescence_Spectra.xlsx) in the article titled “pH-Responsive supramolecular vesicles for imaging-guided drug delivery: Harnessing aggregation-induced emission”.

Description of the data and file structure

1. DLS_data.xlsx:

DLS particle size distribution data:

• Samples: G1 (Figure S10b) and G2 (Figure S10d), H-G1 (Figure 2c) and H-G2 (Figure 2d)
• Measurements: Five repeat measurements for each sample
• Conditions: [H]/[G1] = 1:1, [H]/[G2] = 4:1, [G1] = [G2] = 7.82 × 10–4 M.

Variables:

• Sample Name (Size): Name for each sample for size distribution measurement and the measurement run number (e.g., G1 (1): G1 for the first measurement; H-G1 (5): H-G1 for the fifth measurement)
• T (℃): Temperature in Celsius during measurement
• Z-Average (d. nm): Z-Average particle size in nanometers, derived from the cumulants analysis of the DLS data
• PDI: Polydispersity index, a measure of the width of the particle size distribution
• Size1 (d.nm): Particle size in nanometers for the first measurement
• Intensity-1: Intensity for the first measurement
• Size2 (d.nm): Particle size in nanometers for the second measurement
• Intensity-2: Intensity for the second measurement
• Size3 (d.nm): Particle size in nanometers for the third measurement
• Intensity-3: Intensity for the third measurement
• Size4 (d.nm): Particle size in nanometers for the fourth measurement
• Intensity-4: Intensity for the fourth measurement
• Size5 (d.nm): Particle size in nanometers for the fifth measurement
• Intensity-5: Intensity for the fifth measurement

Zeta potential data:

• Samples: H-G1 (Figure S11a) and H-G2 (Figure S11b)
• Measurements: Five repeat measurements
• Conditions: [H]/[G1] = 1:1, [H]/[G2] = 4:1, [G1] = [G2] = 7.82 × 10–4 M in water.

Variables:

• Sample Name (Zeta): Each sample name for Zeta potential measurement and run number (e.g., H-G1 (1): zeta potential of H-G1 for the first measurement)
• T (℃): Temperature in Celsius during measurement
• Zeta Potential (mV): Zeta potential in millivolts
• Mob (umcm/Vs): Electrophoretic mobility in micrometers per centimeter per volt per second
• Cond (mS/cm): Conductivity in millisiemens per centimeter
• Zeta Potential-1(mV): Zeta potential in millivolts for the first measurement
• Intensity-1: Intensity for the first measurement
• Zeta Potential-2(mV): Zeta potential in millivolts for the second measurement
• Intensity-2: Intensity for the second measurement
• Zeta Potential-3(mV): Zeta potential in millivolts for the third measurement
• Intensity-3: Intensity for the third measurement
• Zeta Potential-4(mV): Zeta potential in millivolts for the fourth measurement
• Intensity-4: Intensity for the fourth measurement
• Zeta Potential-5(mV): Zeta potential in millivolts for the first measurement
• Intensity-5: Intensity for the fifth measurement

2. Figure_4_Fluorescence_Spectra.xlsx:

Raw Fluorescence Data for Figure 4 in the Article:

(a) Absorption and fluorescence emission spectra of G2 and DOX.

(b) Fluorescence spectra of H-G2 vesicles in the presence of different amounts of DOX (λex = 384 nm).

(c) Fluorescence recovery of G2 after adjusting the pH of DOX-loaded H-G2 vesicles (G2+H+DOX) to 4.0 (λex = 384 nm).

(d) Fluorescence recovery of DOX after adjusting the pH of DOX-loaded H-G2 vesicles (G2+H+DOX) to 4.0 (λex = 469 nm) ([H]/[G2] = 4:1, [G2] = 1.0 mM).

Variables:

Figure 4a:

• Wavelength/nm-1: Wavelength in nanometers for the absorbtion of G2
• Ab-G2: Absorbance values for G2
• Wavelength/nm-2: Wavelength in nanometers for the fluorescence emmision of G2
• Em-G2: Fluorescence emission values for G2
• Wavelength/nm-3: Wavelength in nanometers for the absorbtion of DOX
• Ab-DOX: Absorbance values for DOX
• Wavelength/nm-4: Wavelength in nanometers for the fluorescence emmision of DOX
• Em-G2: Fluorescence emission values for DOX

Figure 4b:

• Wavelength/nm: Wavelength in nanometers
• Intensity-0: Fluorescence intensity of H-G2 vesicles in the presence of 0 equivalent of DOX
• Intensity-0.1: Fluorescence intensity of H-G2 vesicles in the presence of 0.1 equivalent of DOX
• Intensity-0.25: Fluorescence intensity of H-G2 vesicles in the presence of 0.25 equivalent of DOX
• Intensity-0.40: Fluorescence intensity of H-G2 vesicles in the presence of 0.40 equivalent of DOX
• Intensity-0.50: Fluorescence intensity of H-G2 vesicles in the presence of 0.50 equivalent of DOX
• Intensity-0.65: Fluorescence intensity of H-G2 vesicles in the presence of 0.65 equivalent of DOX
• Intensity-0.75: Fluorescence intensity of H-G2 vesicles in the presence of 0.75 equivalent of DOX
• Intensity-0.90: Fluorescence intensity of H-G2 vesicles in the presence of 0.90 equivalent of DOX

Figure 4c:

• Wavelength/nm: Wavelength in nanometers
• G2+H+DOX: Fluorescence emmision intensity of DOX-loaded H-G2 vesicles (λex = 384 nm)
• G2:  Fluorescence emmision intensity of G2 (λex = 384 nm)
• pH 4.0 release:  Fluorescence emmision intensity of DOX-loaded H-G2 vesicles after the pH was adjusted to 4.0 (λex = 384 nm)

Figure 4d:

• Wavelength/nm: Wavelength in nanometers
• G2+H+DOX: Fluorescence emmision intensity of DOX-loaded H-G2 vesicles (λex = 469 nm)
• pH 4.0 release:  Fluorescence emmision intensity of DOX-loaded H-G2 vesicles after the pH was adjusted to 4.0 (λex = 469 nm)