Data for: Assessment of genetic diversity and protein content of Scandinavian peas (Pisum sativum)
Data files
Sep 12, 2023 version files 115.88 KB
Abstract
We produced homogeneous lines of 227 pea accessions from the Nordic Genetic Resource Center by single seed descent. The genetic diversity among these, mostly Scandinavian accessions, was investigated using three microsatellite markers, A9, AC58 and AA5. The microsatellites were highly informative and separated 153 of 194 accessions on a Neighbor Joining topology. The high polymorphism information content (PIC) values between 0.87 and 0.91 indicated that the gene bank material contains a large number of pea accessions with different breeding. The peas were grown in the field for two years and seed protein content showed variation between 9.3% and 34.1% over years and accessions. Mean thousand seed weight was 152.05 g. More than 10 accessions had protein content above 28% showing that the collection has potential as a breeding nursery for high-protein pea.
README: Data for: Assessment of genetic diversity and protein content of Scandinavian peas (Pisum sativum)
Authors: Louise Winther, Søren Kjærsgaard Rasmussen, Gert Poulsen and Conny Bruun Asmussen Lange
Description of the Data and file structure
This dataset contains two Excel sheets providing information about SSR-results of the pea accessions as well as protein data.
File: Binary microsatellite.xlsx
A provides accession names. The name is split in 3; the first part is accession number, the second is the name in Nordgen and the third is the name of cultivar.
Row 1 provides length of fragments, with first letter corresponding to color of microsatellite.
Results in cells are binary. 0=No fragment, 1=fragment.
The results are color-coded according to color of microsatellite. Darker rows indicate no fragment present of the accession for the given microsatellite (Blue, Green or Yellow).
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File: Overview of Data.xlsx
A: Accession name/number
B: Nordgen name
C: Cultivar name. Empty cells represent that no cultivar name is available.
D-F: Microsatellite results. x indicates a microsatellite result, Empty cells represent no result from given microsatellite.
G: Thousand seed Weight in gram, weighed in 2017. Empty cells indicate that TSW is not applicable.
H-J: Protein content calculated for 2017, 2018 and 2019. Empty cells indicate that the protein content has not been calculated for the given accession the given year.
Yellow highlights mark accessions which has no microsatellite result (No x in Column D-F), and is thus not included in the genetic analysis, only in protein content.
Sharing/access Information
Article available: https://doi.org/10.3390/agronomy13092307
Data is original, from no other source.