Physiology data for: Biomechanical origins of proprioceptor feature selectivity and topographic maps in the Drosophila leg

Mamiya, Akira, University of Washington, https://orcid.org/0000-0002-3199-5341

Sustar, Anne, University of Washington

Siwanowicz, Igor, Janelia Farm Research Campus

Qi, Yanyan, Baylor College of Medicine

Lu, Tzu-Chiao, Baylor College of Medicine

Gurung, Pralaksha, University of Washington

Chen, Chenghao, University of Washington

Phelps, Jasper, Harvard Medical School

Kuan, Aaron, Harvard Medical School

Pacureanu, Alexandra, European Synchrotron Radiation Facility

Lee, Wei-Chung, Harvard Medical School

Li, Hongjie, Baylor College of Medicine

Mhatre, Natasha, Western University

Tuthill, John, University of Washington

akiram@uw.edu

Published Aug 01, 2023 on Dryad. https://doi.org/10.5061/dryad.dbrv15f6q

Cite this dataset

Mamiya, Akira et al. (2023). Physiology data for: Biomechanical origins of proprioceptor feature selectivity and topographic maps in the Drosophila leg [Dataset]. Dryad. https://doi.org/10.5061/dryad.dbrv15f6q

Abstract

Our ability to sense and move our bodies relies on proprioceptors, sensory neurons that detect mechanical forces within the body. Because they are located within complex and dynamic peripheral tissues, the underlying mechanisms of proprioceptor feature selectivity remain poorly understood. Using single-nucleus RNA sequencing, we found that proprioceptor subtypes in the Drosophila leg express similar complements of mechanosensory and other ion channels. However, anatomical reconstruction of the proprioceptive organ and connected tendons revealed major biomechanical differences between proprioceptor subtypes. We constructed a computational model that identified a biomechanical mechanism for joint angle selectivity and predicted the existence of a goniotopic map of joint angle among position-tuned proprioceptors, which we confirmed using calcium imaging. Our findings suggest that biomechanical specialization is a key determinant of proprioceptor feature selectivity in Drosophila. The discovery of proprioceptive maps in the fly leg reveals common organizational principles between proprioception and other topographically organized sensory systems.

Funding

Janelia Research Campus, Visiting Scientist

Kinship Conservation Fellows

Klingenstein-Simons

Pew Charitable Trusts, Biomedical Scholar Award

McKnight Foundation, Scholar Award

Alfred P. Sloan Foundation, Research Fellowship

New York Stem Cell Foundation

Natural Sciences and Engineering Research Council, Award: 687216

Natural Sciences and Engineering Research Council, Award: 675248

Natural Sciences and Engineering Research Council, Award: 693206

National Institutes of Health, Award: R01NS102333

National Institutes of Health, Award: U01NS115585

National Institutes of Health, Award: R01NS108410

National Institutes of Health, Award: RF1MH128949

National Institutes of Health, Award: R00AG062746

Edward R. and Anne G. Lefler Center

Goldenson Family

Cancer Prevention and Research Institute of Texas, Award: RR200063, Scholar in Cancer Research

The Longevity Impetus Grant

Welch Foundation

Ted Nash Long Life Foundation

European Research Council, Award: 852455, European Union’s Horizon 2020 Research and Innovation Programme

New York Stem Cell Foundation, Robertson Investigator