Responsiveness of the broad bean weevil Bruchus rufimanus Boh. to different Vicia faba L. genotypes
Data files
Apr 22, 2024 version files 242.98 KB
Abstract
The broad bean weevil Bruchus rufimanus Boh., also called bruchid beetle, is a major pest of Vicia faba L. given that larvae develop inside the seeds causing direct quality losses in agricultural products. Recurrent chemical applications are inappropriate because they represent a serious threat to pollinators in general. In addition, biological control approaches that have been attempted so far showed limited effectiveness, which makes studies of methods to control bruchid populations extremely important. Breeding resistant cultivars is the most adequate approach to achieve efficient levels of pest resistance and promote sustainable agriculture. To explore the mechanisms underlying bruchid resistance, we explored B. rufimanus behavior in different life stages and plant preference in feeding, oviposition and larval survival into adulthood both in greenhouse experiments and in the field. Our greenhouse results showed that females, compared to males, have preferences for flowers from some genotypes over the others although the choices made did not correspond with the laying preference. The egg laying preference of females was significantly affected by cultivars. This attraction could be explained by the number of seeds per pod produced by the genotypes, while in the field flowering time influenced bruchid infestation. Moreover, the survival of the larva into adulthood was greater in certain genotypes over the others. The results of our study lay the groundwork for further analyses to finely dissect V. faba resistance towards bruchids and pave the way for the development of methods to predict potential resistant genotypes in research and breeding programs.
README
README
This readme file was generated on 12th September 2022 by Denise Dalbosco Dell'Aglio
GENERAL INFORMATION
Title:
Responsiveness of the broad bean weevil Bruchus rufimanus Boh. to different Vicia faba L. genotypes
Authors:
Denise Dalbosco Dell'Aglio
Nadim Tayeh
Affiliation:
Agrocologie, INRAE, Institut Agro, Univ. Bourgogne Franche-Comt, 21000, Dijon, France
Corresponding author:
Denise Dalbosco Dell'Aglio
email: denise.ddd3@gmail.com
DATA & FILE OVERVIEW
File dataset list:
Ovip_16July2021.txt
Ovip_4dates_5accessions.txt
data_field_16march2022.txt
2Choices_July2021.txt
Adult_18Jan2022.txt
1Choice_July2021.txt
2choices_bysex.txt
File R script:
script_bruchid_manusc.R
METHODOLOGICAL INFORMATION
Description of methods used for collection/generation of data:
Behaviour data collected from live B. rufimanus adults under controlled conditions.
Plant phenological traits collected in the field.
Seed infestation data collect in laboratory conditions.
Methods for processing the data:
All data collected was handled using R software.
DATA SPECIFIC INFORMATION
The data files contains all necessary to be upload into R environment. The R script contains the statistical models, together with each data file to be used and graphs created for the manuscript. More specific details in the R script.
Abbreviations and Variable descriptions:
File Ovip_16July2021.txt: "Name" (name of the Faba type), "Rep" (replica), "Date" (dd/mm/yyyy), "Branch" (number of the plant branch), "Pod.date" (date that the pod start growing, dd/mm/yyyy), "Pod.age" (number of days since Pod.date), "N.eggs" (number of eggs), "New" (if the eggs are new, n for yes)
File Ovip_4dates_5accessions: "Name" (name of the Faba type), "Rep" (replica), "Date" (dd/mm/yyyy), "Branch" (number of the plant branch), "Pod.date" (date that the pod start growing, dd/mm/yyyy), "Pod.age" (number of days since Pod.date), "N.eggs" (number of eggs), "New" (if the eggs are new, n for yes)
File data_field_16march2022.txt: "GPID" (genotype of Faba bean), "Rep" (replica), "Flw_time" (Floweration time in days), "Plant_hm" (Plant height mean in cm), "Seed_100w" (weight of 100 seeds in g), "Seeds_htyp" (percentage of healthy seeds)
File 2Choices_July2021.txt: "choice.name" (name of the choice pair), "choice.A" (name of first choice), "choice.B" (name of second choice), "sex" (if male or female), "status" (naive, never fed before; mature, already fed; all, both naive and mature data together), "ind" (number of individuals), "result.A" (number of individuals which choose the choice A), "result.B" (number of individuals which choose the choice B), "prop.A" (proportion of choice A), "prop.B" (proportion of choice B)
File Adult_18Jan2022.txt: "name" (name of faba genotype), "rep" (replica), "branch" (number of the plant branch), "pod_date" (date that the pod start growing, dd/mm/yyyy), "pod_age" (number of days since Pod.date), "eggs_n" (number of eggs), "seeds_n" (total number of seeds), "infested_n" (number of infested seeds), "holes_n" (total number of holes in the seeds), "seeds_weight" (total weight of the seeds in g), "holes_seed" (number of holes per seed), "infested_per" (percentage of infested seeds), "oneseed_weight" (the weight of 1 seed), "eggs_seed" (number of eggs per seed)
File 1Choice_July2021.txt: "choice.name" (name of the choice pair), "choice.A" (name of first choice), "choice.B" (name of second choice), "sex" (if male or female), "status" (naive, never fed before; mature, already fed; all, both naive and mature data together), "n_ind" (number of individuals), "result.A" (number of individuals which choose the choice A), "result.B" (number of individuals which choose the choice B), "total" (total number of individuals which made a choice), "prop.A" (proportion of choice A), "prop.B" (proportion of choice B), "signf" (if the difference between A and B is statistically significant, y for yes, n for no)
File 2choices_bysex.txt: "choice.name" (name of the choice pair), "GPID" (genotype of Faba bean), "sex" (if male or female), "n_ind" (number of individuals which made the choice), "prop" (proportion of individuals which made a choice)
Methods
1. Description of methods used for collection/generation of data:
Behaviour data collected from live B. rufimanus adults under controlled conditions.
Plant phenological traits collected in the field.
Seed infestation data collect in laboratory conditions.
2. Methods for processing the data:
All data collected was handled using R software.
Usage notes
R software