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Dryad

Effects of phloretin, ritonavir, indinavir and Bay-876 on glucose transport during peritoneal dialysis in Sprague-Dawley rats

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Aug 07, 2024 version files 148.89 KB

Abstract

We previously found that phloretin, a general blocker of facilitative glucose channels (GLUTs), reduced glucose absorption and improved ultrafiltration during peritoneal dialysis (PD) in rats. It is as yet unknown via which GLUTs such transport occurs and whether transport occurs in a trans-cellular fashion. This study was undertaken to elucidate (i) whether glucose may be transferred in a trans-cellular fashion across peritoneal cells and (ii) via which GLUTs glucose transport may be partly mediated during PD. The main study was performed in thirty-two Sprague-Dawley rats in which PD was performed by instillation of 20 mL 2.3% glucose PD fluid which was allowed to dwell for 60 min. Animals were given an IV infusion of [18F]-deoxyglucose allowing us to monitor glucose transport in both directions: from the dialysate to the circulation and vice versa. Animals received either no treatment (control), or received intra-peritoneal phloretin (50 mg/L), ritonavir (60 mg/L), or BAY-876 (25 mg/L). Water and transfer of low-molecular solutes were closely monitored during dialysis. Additional experiments were then performed in sixteen rats using receiving either no treatment (control) or intra-peritoneal indinavir (50 mg/L).