Quantitative disease resistance in wild Silene vulgaris to its endemic pathogen Microbotryum silenes-inflatae collection sites
Data files
Nov 23, 2023 version files 9.83 KB
Abstract
Details presented on the collections sites of Silene vulgaris seeds from natural populations, for use in the study, Quantitative Disease Resistance in wild Silene vulgaris to its Endemic Pathogen Microbotryum silenes-inflatae. In this study the seeds were collected a mixed half and full sibling families (seeds from the same maternal plants) and used to produce plants that were then cloned by vegetative cuttings. The infection rates of these clones, as well as of their F1 offspring, by the fungus Microbotryum silenes-inflatae are also presented.
README: Quantitative Disease Resistance in wild Silene vulgaris to its Endemic Pathogen Microbotryum silenes-inflatae Collection Sites
https://doi.org/10.5061/dryad.fj6q57426
The data describe the collection sites and production of cloned offspring for the plant Silene vulgaris..
Description of the data and file structure
The information is provided in three cvs tables, found on three files as untransformed data.
The first file (titled: Field Sites for Seed Collection) contains the plant families from collection sites identified by the corresponding names in the column "field-collected family IDs". The field-collected families each represent mixtures of full and half-sibling groups as the seeds collected from the same maternal plant. The family IDs were given alphanumeric, single character names ranging from A-Z and 0-9, where families with sufficient seeds were included in the current study. Seeds from these families, representing individual plant genotypes, were used to produce clones by vegetative propagation. In addition, the "Number of Genotypes Cloned" the "Average Number of Clones per Genotype" and standard deviation are given.
The second file (titled: Infect. Field Collect Families) includes the plant families identified by the corresponding names in the column "Field-Collected Family ID", that are the same as the family names in first data file (titled: Field Sites for Seed Collection); field-collected families each represent mixtures of full and half-sibling groups as the seeds collected from the same maternal plant. The family IDs were given alphanumeric, single character names ranging from A-Z and 0-9, where families with sufficient seeds were included in the current study. The "Genotype ID within Family" representing the plant derived from an individual seed, "Clone Count per Genotype", "Number Infected" indicating the number of clones that became diseased following inoculation, and "Proportion Diseased" among those clones.
The third file (titled: Infec F1 Offspring) includes "Replicate", "Resistance Quartile of Maternal Parent", "Maternal Family", "Maternal Parent Genotype", "F1 Offspring Count", "Number Infected", and "Proportion Diseased". These data show the results of the infection experiment with the F1 offspring from field-collected families shown in the first and second data files (titled: Field Sites for Seed Collection, and titled: Infect. Field Collect Families). "Replicate" indicates the two replicates runs of the experiment. The maternal parents of the F1 offspring were ranked according to their susceptibility to infection (as shown in the second worksheet, titled: Infect. Field Collect Families), and grouped into ranked resistance quartiles that are indicated by "Resistance Quartile of Maternal Parent" with value of 1 as the most resistant and 4 as the most susceptible. "Maternal Family" corresponds to the field-collected family to which the maternal parent of the F1 offspring belonged (identified in the second data file titled: Field-Collected Family ID); field-collected families each represent mixtures of full and half-sibling groups as the seeds collected from the same maternal plant. "Maternal Parent Genotype" corresponds to a combination code that adds together the "Maternal Family" and the number identifying the plant derived from an individual seed, shown as the "Genotype ID within Family" from the second worksheet (titled: Field-Collected Family ID). "F1 Offspring Count" shows the number of F1 offspring that were included in inoculation experiment. "Number Infected" indicates the number of F1 offspring that became diseased following inoculation. "Proportion Diseased" shows the number that became infected divided by the number of F1 offspring that were included in the study.
Sharing/Access information
These data are associated with the in-progress publication "Quantitative Disease Resistance in wild Silene vulgaris to its Endemic Pathogen Microbotryum silenes-inflatae."
Methods
Surveys of natural populations of Silene vulgaris identified sites of seed collection. Data on the susceptibility to the fungal pathoge, Microbotryum silenes-inflatae, were collected from greenhouse experiments, including the susceptiblity of the field-collected seed genotypes based on growing the plants and producing multiple clones (by vegetative propogation) prior to inoculation (data show the proportion of clones that became diseased), and including susceptibility of F1 generation produced in the greenhouse from those genotypes and then inoculating the offspring (data showing the proportion of F1 offspring that became diseased). Data presented are not transformed.