Ectopic expression of murine CD163 enables cell-culture isolation of lactate dehydrogenase-elevating (LDV) virus 63 years after its discovery
Data files
Apr 23, 2024 version files 26 KB
Abstract
Arteriviruses are RNA viruses related to coronaviruses but have not yet been associated with human infection. A murine arterivirus (lactate dehydrogenase-elevating virus, LDV) was first described in 1960 and quickly became a promising model for understanding immune failure due to its unique ability to persist in immunocompetent adult mice. However, inability to culture LDV in vitro ultimately limited this system. Here, we demonstrate that the macrophage marker CD163 is essential for LDV infection. Expression of the murine homolog (mCD163) in otherwise mCD163-negative cell lines from mice and nonhuman primates enables productive LDV infection, creating the first immortalized cell-culture system. We also show that mCD163-knockout mice are completely resistant to LDV infection. These findings advance LDV as a model of arterivirus infection viral persistence and add to a growing body of literature suggesting that CD163 utilization is a broad feature of arteriviruses.
README: Ectopic expression of murine CD163 enables cell-culture isolation of lactate dehydrogenase-elevating (LDV) virus 63 years after its discovery
Raw data for plaque assay and qPCR experiments performed as part of this publication.
Description of the data and file structure
Each tab in the .csv file corresponds to the data from a figure.
1A: shows LDV N-gene copies per mL of supernatant generated from RT-qPCR with an RNA standard curve, expressed as a Log10 value, for 6 different cell types, with 3 replicates.
1B: Shows RT-qPCR data for cell types shown in 1A measuring CD163 and beta-acin, expressed as raw Ct value.
2B: shows LDV N-gene copies per mL of supernatant generated from RT-qPCR with an RNA standard curve, expressed as a Log10 value, for different cell types with or without ectopic CD163, with 3 replicates.
2C: LDV N-gene copies per mL of supernatant generated from RT-qPCR with an RNA standard curve, expressed as a Log10 value, versus LDV plaque forming units, in triplicate.
3E: LDV strains p, c, and w plaque forming units per mL of supernatant generated on 3T3 cells with and without CD163 ectopic expression.
4C: LDV strains p, c, and w plaque forming units per mL of supernatant generated on MA-104 cells with and without CD163 ectopic expression.
5B: LDVp viral loads, shown as N-gene copies per mL or plaque forming units per mL, on primary peritoneal macrophages.
5C: LDVp N-gene copies per mL of serum generated from RT-qPCR with an RNA standard curve, expressed as a Log10 value, in CD163 knockout or wild-type mice.
5D: LDV strains p, c, and w plaque forming units per mL of serum in CD163 knockout or wild-type mice.
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Published in the Journal of Virology.
Data was derived from the following sources:
Laboratory of Adam Bailey at UW-Madison