Description of the data and file structure
(1) Fig.3e-Fluorescence_enhancement_factor.csv
This document is the enhancement factor of fluorescent FDG, Cyanine3, and Tex-red inside Flat-Chip, Solid-Chip, and NC-Chip. Before use, the FDG solution at FDG: β-Gal ratio of 1:1 was created and reacted for 30 min at 37 °C in the dark. The enhancement factor was calculated by ∆E,exp = (IE−Ix)/(I0−Ix), where IE represents the intensity on the NC-Chip or Solid-Chip on the herringbone pattern, I0 represents the intensity on the Flat-Chip channel, and Ix represents the background correction. The FDG represents fluorescein-di-β-d-galactopyranoside. The β-Gal represents β-galactosidase. The NC-Chip represents nanopore-crystal microchip. The Solid-Chip represents solid herringbone chip. The Flat-Chip represents an assay chip on clean glass slides.

(2) Fig.3f-Simulated_reflection_spectra.csv
This document is the simulated reflection spectra of NC-Chip excited by plane light. The N represents the layer of nanopore-crystal structure.

(3) Fig.3h-Simulated_fluorescence_emission_power_curves.csv
This document is the simulated fluorescence emission power curves of a dipole on a glass substrate (Flat chip) and nanopore-crystal structure (NC-Chip). A single dipole was employed to simulate the generated fluorescence of FDG.

(4) Fig.4d-Capture_efficiency_of_anti-CD81-based_NC-Chip_and_Solid-Chip.csv
This document is the comparison capture efficiency of anti-CD81-based NC-Chip and Solid-Chip using DiO-stained PANC-1 Exo (1.0×109 particles mL-1) in PBS and extracellular vesicles-depleted plasma (100-fold dilution). The anti-CD81-based NC-Chip and Solid-Chip represents the NC-Chip and Solid-Chip that was modified by CD81 monoclonal antibodies. The DiO represents 3,3'-dioctadecyloxacarbocyanine perchlorate. The PANC-1 Exo represents PANC-1 cell-derived exosomes.

(5) Fig.4e-Detectionand_Solid-Chip_dynamic_range_for_quantifying_NCL_expression on_PANC-1_Exo.csv
This document is the detection dynamic range for quantifying nucleolin (NCL) expression on PANC-1 Exo by the NC-Chip and Solid-Chip. The c represents the concentration of exosomes.

(6) Fig.4f-NC-Chip_for_detection_of_NCL_expression.csv
This document is the NC-Chip for detection of NCL expression on hTERT-HPNE cell-derived exosomes (hTERT-HPNE Exo) and PANC-1 Exo (1.0 × 109 particles mL-1) spiked in both PBS and 100-fold diluted extracellular vesicles (EV)-depleted plasma. Control experiment for PBS or 100-fold diluted EV-depleted plasma.

(7) Fig.4g-Calibration_curves_for_detection_of_NCL_expression.csv
This document is the calibration curves for detecting the NCL expression on PANC-1 Exo spiked in PBS and 100-fold diluted EV-depleted plasma.

(8) Fig.4h-Radar_plot_showing _expression_of eight proteins_from_one_normal_and _seven_tumor_cell_lines.csv
This document is the radar plot showing expression of eight proteins from one normal hTERT-HPNE- and seven tumor PC cell lines (CFPAC-1-, PANC-1-, MIAPaCa-2-, SW1990, Panc 08.13, PaTu 8988t, and BxPC-3)-derived exosomes.

(9) Fig.4i-1-Correlation_of_expression_of_eight_proteins_on_unpurified_Exo.csv
This document is the correlation of expression of eight proteins on unpurified Exo derived from PANC-1-, MIAPaCa-2-, and SW1990 cell lines by NC-Chip. The unpurified Exo represents a cell-depleted culture medium.

(10) Fig.4i-2-Correlation_of_expression_of_eight_proteins_on_UC-purified_Exo.csv
This document is the correlation of expression of eight proteins on ultracentrifugation (UC)-purified Exo derived from PANC-1-, MIAPaCa-2-, and SW1990 cell lines by NC-Chip.

(11) Fig.4j-NC-Chip_assessment_of_tExos_in_PR_control_and_two_PC_patient_plasma.csv
This document is the NC-Chip assessment of eight protein expression levels on Tumor-derived exosomes (tExos) directly from one pancreatitis (PR) control and two pancreatic cancer (PC) patient plasma samples.

(12) Fig.5-Integrative_phenotyping_of_pancreatic_cancer_exosomes_in_a_training _cohort.csv
This document is the integrative phenotyping of pancreatic cancer exosomes in a training cohort. A training set of 28 age-matched healthy donors (HD), 26 PR, 40 non-metastatic pancreatic cancer (nPC), and 25 metastatic pancreatic cancer (mPC). After removing the background, the signal intensities for each sample were averaged over three measurements and then normalized using min-max. The NA represents represents the word "information not available."

(13) Fig.6-Validation_of_the_NC-Chip_for_PC_diagnosis.csv
This document is the validation of the NC-Chip for PC diagnosis. A validation set of 11 HD, 26 PR, 33 nPC, and 20 mPC participants. After removing the background, the signal intensities for each sample were averaged over three measurements and then normalized using min-max.