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Dryad

Data from: SUB-immunogold-SEM reveals nanoscale distribution of submembranous epitopes

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Aug 26, 2024 version files 1.64 GB

Abstract

Electron microscopy paired with immunogold labeling is the most precise tool for protein localization. However, these methods are either cumbersome, resulting in small sample numbers and restricted quantification, or limited to identifying protein epitopes external to the membrane. Here, we introduce SUB-immunogold-SEM, a scanning electron microscopy technique that detects intracellular protein epitopes proximal to the membrane. We identified four critical sample preparation factors that contribute to the method’s sensitivity and validated its efficacy through precise localization and high-powered quantification of cytoskeletal and transmembrane proteins. We evaluate the capabilities of SUB-immunogold-SEM on cells with highly differentiated apical surfaces: (i) auditory hair cells, revealing the presence of nanoscale MYO15A-L rings at the tip of stereocilia; and (ii) respiratory multiciliate cells, mapping the distribution of the SARS-CoV-2 receptor ACE2 along the motile cilia. SUB-immunogold-SEM extends the application of SEM-based nanoscale protein localization for the first time to intracellular epitopes on the exposed surfaces of any cell.