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CRISPR/Cas9 knock-out sgRNA sequencing in human CaCo2 cells


Vogel, Georg-Friedrich (2023), CRISPR/Cas9 knock-out sgRNA sequencing in human CaCo2 cells, Dryad, Dataset,


The data was obtained in course of performing a CRISPR/Cas9 knock-out in human polarized epithelial cells (CaCo2). The screen aimed to identify genes relevant for proper epithelial polarization and correct apical transport of the apical transmembrane protein dipeptidyl-peptidase 4. It includes next-generation-sequencing data (raw sgRNA-read-counts) of two experimental (sorted) and corresponding control (ctrl) cell populations. It further contains sequence information on the sgRNAs as represented in the human GeCKOv2-CRISPR library and the corresponding targeted genes.


Austrian Science Fund

Oesterreichische Nationalbank

Österreichischen Akademie der Wissenschaften