Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects

van Teeffelen, Sven, Institut Pasteur, Microbial Morphogenesis and Growth Laboratory, Institut Pasteur, Paris, France, https://orcid.org/0000-0002-0877-1294

Vigouroux, Antoine, Institut Pasteur, Microbial Morphogenesis and Growth Laboratory, Institut Pasteur, Paris, France, https://orcid.org/0000-0002-8398-5073

Cordier, Baptiste, Microbial Morphogenesis and Growth Laboratory, Institut Pasteur, Paris, France, https://orcid.org/0000-0002-6042-9787

Aristov, Andrey, Microbial Morphogenesis and Growth Laboratory, Institut Pasteur, Paris, France

Alvarez, Laura, Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Department of Molecular Biology, Umeå University, Umeå, Sweden

Özbaykal, Gizem, Microbial Morphogenesis and Growth Laboratory, Institut Pasteur, Paris, France

Chaze, Thibault, Proteomics Platform, Institut Pasteur, Paris, France

Oldewurtel, Enno Rainer, Microbial Morphogenesis and Growth Laboratory, Institut Pasteur, Paris, France

Matondo, Mariette, Proteomics Platform, Institut Pasteur, Paris, France

Cava, Felipe, Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Department of Molecular Biology, Umeå University, Umeå, Sweden

Bikard, David, Synthetic Biology Laboratory, Institut Pasteur, Paris, France

sven.vanteeffelen@gmail.com, antoine.vigouroux@pasteur.fr

Published Jan 06, 2020 on Dryad. https://doi.org/10.5061/dryad.m37pvmcxq

Cite this dataset

van Teeffelen, Sven et al. (2020). Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects [Dataset]. Dryad. https://doi.org/10.5061/dryad.m37pvmcxq

Abstract

Cell shape and cell-envelope integrity of bacteria are determined by the peptidoglycan cell wall. In rod-shaped Escherichia coli, two conserved sets of machinery are essential for cell-wall insertion in the cylindrical part of the cell: the Rod complex and the class-A penicillin-binding proteins (aPBPs). While the Rod complex governs rod-like cell shape, aPBP function is less well understood. aPBPs were previously hypothesized to either work in concert with the Rod complex or to independently repair cell-wall defects. First, we demonstrate through modulation of enzyme levels that aPBPs do not contribute to rod-like cell shape but are required for mechanical stability, supporting their independent activity. By combining measurements of cell-wall stiffness, cell-wall insertion, and PBP1b motion at the single-molecule level, we then present evidence that PBP1b, the major aPBP, contributes to cell-wall integrity by repairing cell wall defects.

Usage notes

- Tracking dataset: Each file is a csv (tab-separated) file with the X and Y positions of the particles, and the unique particle ID for each track. See ReadMe file.

- SDS Page raw images. See ReadMe file.

Funding

European Research Council, Award: 679980

European Research Council, Award: 677823

Agence Nationale de la Recherche, Award: ANR-10-LABX-62-IBEID

Volkswagen Foundation

Knut and Alice Wallenberg Foundation

Swedish Research Council

Kempe Foundation

Mairie de Paris, Award: Emergence(s)

Laboratory for Molecular Infection Medicine Sweden