Cold tolerance of biological control agents Amblydromalus limonicus and Amblyseius degenerans
Data files
Aug 14, 2024 version files 56.15 KB
Abstract
Knowledge about cold tolerance of non-native biological control agents is critical to avoid permanently establishing them in new temperate areas. The cold tolerance of the predatory mites, A. limonicus and A. degenerans, was investigated in the laboratory to assess their establishment potential in northern Europe, particularly Sweden. The lethal time of A. degenerans declined steeply from 5°C to 0°C and was almost zero at -5°C. The lethal time of A. limonicus did not differ between 5°C and 0°C but was reduced at -5°C. For both species, LTime50 was longer for fed than for unfed mites. In the lethal temperature experiments, a temperature as low as -17.75°C was required to achieve a 100% mortality rate for A. limonicus, whereas most A. degenerans died at -8.5°C. LTemp50 was lower for A. limonicus (-9.8°C) than for A. degenerans (-0.1°C). Collectively, these findings suggest that A. degenerans is unlikely to be established in Sweden but that A. limonicus is more cold-tolerant. This highlights the risk associated with releasing A. limonicus in Sweden, due to concerns about potential establishment.
README: Cold tolerance of biological control agents Amblydromalus limonicus and Amblyseius degenerans
This readme file describes the contents of 14 .txt files used to perform the analyses. Information about how the experiments were conducted can be found in the associated manuscript.
Description of the data and file structure
List of contents:
- Lethal time degenerans.txt
- Lethal time limonicus.txt
- Lethal temperature degenerans.txt
- Lethal temperature limonicus.txt
- LTime50_deg_5fed.txt
- LTime50_deg_5unfed.txt
- LTime50_lim_5fed.txt
- LTime50_lim_5unfed.txt
- LTime50_lim_0fed.txt
- LTime50_lim_0unfed.txt
- LTime50_lim_-5fed.txt
- LTime50_lim_-5unfed.txt
- LTemp50_deg.txt
- LTemp50_lim.txt
Description of Lethal time degenerans.txt
- Id: individual mite ID in each experiment
- Species: the species being tested (degenerans)
- Batch: the batch number (2 batches in total)
- Temperature: the temperature regimes in the experiments (-5°C, 0°C, 5°C)
- Treatment: the treatment specifying whether mites were fed or unfed
- Number of days: the number of days until mite mortality
Description of Lethal time limonicus.txt
- Id: individual mite ID in each experiment
- Species: the species being tested (limonicus)
- Batch: the batch number (3 batches in total)
- Temperature: the temperature regimes in the experiments (-5°C, 0°C, 5°C)
- Treatment: the treatment specifying whether mites were fed or unfed
- Number of days: the number of days until mite mortality
Description of Lethal temperature degenerans.txt
- Id: individual mite ID in each experiment
- Species: the species being tested (degenerans)
- Replications: the number of replications (5 in total)
- Temperature: the temperature regimes in the experiments (-17.75°C, -13.13°C, -8.5°C, -3.88°C, 0.75°C, 3.88°C, 10°C)
- Mortality: specifies whether a mite died after brief exposure to a given temperature
Description of Lethal temperature limonicus.txt:
- Id: individual mite ID in each experiment
- Species: the species being tested (limonicus)
- Replications: the number of replications (5 in total)
- Temperature: the temperature regimes in the experiments (-17.75°C, -13.13°C, -8.5°C, -3.88°C, 0.75°C, 3.88°C, 10°C)
- Mortality: specifies whether a mite died after brief exposure to a given temperature
Description of LTime50_deg_5fed.txt:
- Species: the species being tested (degenerans)
- Temp_treat: the temperature and treatment combination (5°C and fed)
- Day: the cumulative number of days until all the mites died
- Prop_mortality: the cumulative proportion of mites that died (calculated using Mortality and Total)
- Mortality: the cumulative number of mites that died
- Total: the total number of mites in the experiment
Description of LTime50_deg_5unfed.txt:
- Species: the species being tested (degenerans)
- Temp_treat: the temperature and treatment combination (5°C and unfed)
- Day: the cumulative number of days until all the mites died
- Prop_mortality: the cumulative proportion of mites that died (calculated using Mortality and Total)
- Mortality: the cumulative number of mites that died
- Total: the total number of mites in the experiment
Description of LTime50_lim_5fed.txt:
- Species: the species being tested (limonicus)
- Temp_treat: the temperature and treatment combination (5°C and fed)
- Day: the cumulative number of days until all the mites died
- Prop_mortality: the cumulative proportion of mites that died (calculated using Mortality and Total)
- Mortality: the cumulative number of mites that died
- Total: the total number of mites in the experiment
Description of LTime50_lim_5unfed.txt:
- Species: the species being tested (limonicus)
- Temp_treat: the temperature and treatment combination (5°C and unfed)
- Day: the cumulative number of days until all the mites died
- Prop_mortality: the cumulative proportion of mites that died (calculated using Mortality and Total)
- Mortality: the cumulative number of mites that died
- Total: the total number of mites in the experiment
Description of LTime50_lim_0fed.txt:
- Species: the species being tested (limonicus)
- Temp_treat: the temperature and treatment combination (0°C and fed)
- Day: the cumulative number of days until all the mites died
- Prop_mortality: the cumulative proportion of mites that died (calculated using Mortality and Total)
- Mortality: the cumulative number of mites that died
- Total: the total number of mites in the experiment
Description of LTime50_lim_0unfed.txt:
- Species: the species being tested (limonicus)
- Temp_treat: the temperature and treatment combination (0°C and unfed)
- Day: the cumulative number of days until all the mites died
- Prop_mortality: the cumulative proportion of mites that died (calculated using Mortality and Total)
- Mortality: the cumulative number of mites that died
- Total: the total number of mites in the experiment
Description of LTime50_lim_-5fed.txt:
- Species: the species being tested (limonicus)
- Temp_treat: the temperature and treatment combination (-5°C and fed)
- Day: the cumulative number of days until all the mites died
- Prop_mortality: the cumulative proportion of mites that died (calculated using Mortality and Total)
- Mortality: the cumulative number of mites that died
- Total: the total number of mites in the experiment
Description of LTime50_lim_-5unfed.txt:
- Species: the species being tested (limonicus)
- Temp_treat: the temperature and treatment combination (-5°C and unfed)
- Day: the cumulative number of days until all the mites died
- Prop_mortality: the cumulative proportion of mites that died (calculated using Mortality and Total)
- Mortality: the cumulative number of mites that died
- Total: the total number of mites in the experiment
Description of LTemp50_deg.txt:
- Species: the species being tested (degenerans)
- Temperature: the temperature regimes ranging from 10°C to -17.75°C
- Replication: the number of replications
- Prop_mortality: the proportion of mites that died in each temperature regime (calculated using Mortality and Total)
- Mortality: the number of mites that died in each temperature regime
- Total: the total number of mites in the experiment
Description of LTemp50_lim.txt:
- Species: the species being tested (limonicus)
- Temperature: the temperature regimes ranging from 10°C to -17.75°C
- Replication: the number of replications
- Prop_mortality: the proportion of mites that died in each temperature regime (calculated using Mortality and Total)
- Mortality: the number of mites that died in each temperature regime
- Total: the total number of mites in the experiment
Methods
The dataset was collected during laboratory experiments conducted at the Swedish University of Agricultural Sciences in Uppsala and has been processed by a series of generalized linear models using package stats in R version 4.2.1.