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Dryad

Data for: SUN2 regulates mitotic progression in response to extracellular matrix rigidity

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Nov 09, 2022 version files 46.61 KB

Abstract

How cells adjust their growth to the spatial and mechanical constraints of their surrounding environment is central to many aspects of biology. Here we examined how ECM rigidity affects cell division. We found that cells divide more rapidly when cultured on rigid substrate. While we observed no effect of ECM rigidity on rounding or post-mitotic spreading duration, we found that changes in matrix stiffness impact mitosis progression. We noticed that ECM elasticity up-regulates the expression of the LINC complex component SUN2, which in turn promotes metaphase-to-anaphase transition by acting on mitotic spindle formation. Whereas when cells adhere on soft ECM, low levels of SUN2 expression perturb astral microtubule organization and delay the onset of anaphase.