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Dryad

Gene expression analysis of alveolar macrophages tolerized to ozone

Abstract

In this study, we examined the effect of repeated exposure to the air pollutant ozone (O3) on the transcriptome of alveolar macrophages (AMs). Using flow cytometry, we isolated purified AMs from the lungs of female C57BL6/NJ mice exposed to filtered air or 0.8 ppm O3 for four days and then collected 2 days later, with six mice per group. RNA was isolated from AMs and then subjected to RNA-seq analysis. We performed differential gene expression analysis followed by gene ontology enrichment analysis of differentially expressed genes.