Restoration of striatal neuroprotective pathways by kinase inhibitor treatment of Parkinson’s linked-LRRK2 mutant mice
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Nov 18, 2024 version files 89.89 GB
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Feb 26, 2025 version files 90.15 GB
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Mar 28, 2025 version files 110.33 GB
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Abstract
Parkinson’s disease-associated, activating mutations in Leucine Rich Repeat Kinase 2 (LRRK2) block primary cilia formation in cholinergic and parvalbumin interneurons and astrocytes in the striatum, decreasing the production of GDNF and NRTN neuroprotective factors that normally support dopaminergic neuron viability. We show here that 3 month-dietary administration of the MLi-2 LRRK2 kinase inhibitor restores primary cilia and the Hedgehog-responsive production of neuroprotective GDNF and NRTN by these neurons; cilia are also restored on cholinergic neurons of the pedunculopontine nucleus. Importantly, we detect recovery of striatal dopaminergic processes and decreased stress-triggered Hedgehog signaling by nigral dopaminergic neurons. Thus, pathogenic LRRK2-driven cilia loss is reversible in post-mitotic neurons and astrocytes, which suggests that early administration of specific LRRK2 inhibitors may have significant therapeutic benefit for patients in the future.
https://doi.org/10.5061/dryad.q2bvq83tn
Author/Principal Investigator Information
Name: Suzanne R. Pfeffer
ORCID: 0000-0002-6462-984X
Institution: Stanford University
Address: Beckman
Center Room B413
279 Campus DriveStanford, California 94305-5307
Email: pfeffer@stanford.edu
Author/Principal Investigator Information
Name: Dario R. Alessi
ORCID: 0000-0002-2140-9185
Institution: MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, United Kingdom
Address: MRC PPU
Sir James Black Centre
School of Life Sciences
University of Dundee
Dow St.
Dundee DD1 5EH
Email: d.r.alessi@dundee.ac.uk
Author/Associate or Co-investigator Information
Name: Ebsy Jaimon
ORCID: 0000-0001-6845-2095
Institution: Stanford University
Address: Beckman Center Room B413
279 Campus Drive
Stanford, California 94305-5307
Email: ebsy@stanford.edu
Author/Associate or Co-investigator Information
Name: Yu-En Lin
ORCID: 0000-0002-5848-5405
Institution: Stanford University
Address: Beckman Center Room B413
279 Campus Drive
Stanford, California 94305-5307
Email: yuenlin@stanford.edu
Author/Associate or Co-investigator Information
Name: Francesca Tonelli
ORCID: 0000-0002-4600-6630
Institution: MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, United Kingdom
Address: MRC PPU
Sir James Black Centre
School of Life Sciences
University of Dundee
Dow St.
Dundee DD1 5EH
Email: f.tonelli@dundee.ac.uk
Author/Associate or Co-investigator Information
Name: Odetta Antico
ORCID: 0000-0002-7325-3303
Institution: MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, United Kingdom
Address: MRC PPU
Sir James Black Centre
School of Life Sciences
University of Dundee
Dow St.
Dundee DD1 5EH
Email: o.antico@dundee.ac.uk
Date of data collection: 2023-06-11
Geographic location of data collection: Stanford, CA and University of Dundee, Scotland
Description of the data and file structure
File list:
Fig 1A zip file:
Raw immunoblotting data (.tiff files) and annotated images (.tiff files) for Figure 1A. Littermate- or age-matched LRRK2 R1441C homozygous knock-in mice were fed either a control diet or MLi-2-containing diet for 90 days prior to tissue collection. On the last day of the study, three mice from each group were euthanized by cervical dislocation and tissues (brain and kidneys) collected from these mice were used to monitor inhibition of LRRK2 activity by immunoblotting. 12.5 μg tissue extract was subjected to quantitative immunoblotting analysis with the indicated antibodies and data analyzed using the LI-COR Odyssey CLx imaging and Image Studio software. Each lane represents a tissue sample from a different animal.
Fig 1BCD zip file:
Images of striatal cholinergic interneurons from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow.
Fig 1EFG zip file:
Images of striatal astrocytes from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow. Note that images of Brains 2 and 9 are split into two files.
Figure 1HIJK zip file:
-Raw images from 5-month-old WT and R1441C LRRK2 homozygous knock-in mice. Brains 6,7,8, and 14 are from WT animals, and brains 5,9,12, and 13 are from R1441C LRRK2 homozygous knock-in animals.
-Raw images from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow.
-Tabular data in .csv format with the raw values used to generate Fig 1I and Fig 1K.
- Tabular data and graph in prism format used to generate Figure 1I and Figure 1K.
-Image files used to generate Fig 1H and Fig 1J.
Fig 2ABC zip file:
Images of PPN cholinergic neurons from 4-month-old R1441C LRRK2 homozygous knock-in mice. Brains 1, 2, 5, and 6 are WT, and Brains 3, 4, 7, and 8 are R1441C LRRK2 homozygous knock-in. Note that images of Brains 6 are split into two files.
Fig 2DEF zip file:
Images of PPN cholinergic neurons from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow. Note that images of Brains 10, 11 and 12 are split into two files.
Fig 3ABC zip file:
Images of striatal cholinergic interneurons from 5-month-old WT and G2019S homozygous knock-in mice. Brains 1,4,7,8 are WT and Brains 2,3,5,6 are G2019S LRRK2 homozygous knock-in. Note that images of all brains are split into two files.
Fig 3DEF zip file:
Images of striatal cholinergic interneurons from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow.
Fig 4ABC zip file:
Images of striatal astrocytes from 5-month-old WT and G2019S homozygous knock-in mice. Brains 1,4,7,8 are WT and Brains 2,3,5,6 are G2019S LRRK2 homozygous knock-in.
Fig 4DEF zip file:
Images of striatal astrocytes from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow. Note that images of brains 2, and 9 are split into two files.
Fig 5ABC zip file:
Images of striatal cholinergic interneurons from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow.
Fig 6ABCDE zip file:
Images of striatal parvalbumin interneurons from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow.
Fig 7ABCE zip file:
Images from 5-month-old WT and R1441C LRRK2 homozygous knock-in mice. Brains 6,7,8, and 14 are from WT animals, and brains 5,9,12, and 13 are from R1441C LRRK2 homozygous knock-in animals. Note that images of Brain 8 are split into two files.
Fig 7FGHIK zip file:
Images from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow.
Fig 7DJ zip file:
- Tabular data in .csv format with the raw values used to generate Fig 7D and Fig 7J.
- Tabular data and graph in prism format used to generate Figure 7DJ.
Figure 7I zip file:
-Tabular data in .csv format with raw values used to generate Fig 7I. Automated determination of intensity was carried out using CellProfiler.
-Graph in prism format used to create Fig 7I
Fig 8AB zip file:
Images of nigral dopaminergic neurons from 10-month-old WT and R1441C LRRK2 homozygous knock-in mice. Brains 7, 12, 22, 25, 34 are WT and Brains 9, 23, 33, 36, 38 are R1441C LRRK2 homozygous knock-in.
Fig 8CD zip file:
Images of nigral dopaminergic neurons from R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months. Brains 1, 2, 3, 10, 11, and 12 are from R1441C LRRK2 homozygous knock-in mice fed with control chow and Brains 4, 5, 6, 7, 8, and 9 are from R1441C LRRK2 homozygous knock-in mice fed with MLi-2 inhibitor containing chow.
Raw figure files zip file:
Raw image files used to generate all main figures except Fig 1A.
Raw Fig S1 zip file:
Raw image files used to generate figures in Fig S1. Example images of cilia length.
Raw Fig S2 zip file:
Raw image files used to generate figures in Fig S2. Images of control RNAScope reactions carried out for cholinergic interneurons, astrocytes, parvalbumin interneurons, and nigral dopaminergic neurons.
Raw Fig S3 zip file:
Raw image files used to generate figures in Fig S3. Example images of ciliated and unciliated striatal parvalbumin interneurons.
Raw Fig S4 zip file:
-Raw images used to generate figures in Fig S4A, B, C. Example images of striatum from 5-month-old WT, 5-month-old R1441C LRRK2 homozygous knock-in mice, and R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months.
-Raw immunoblotting data (.tiff files) and annotated images (.tiff files) for Figure S4D, E
Raw Fig S5 zip file:
-Raw genotyping results of R1441C LRRK2 homozygous knock-in mice (10 weeks old) fed with MLi-2 inhibitor containing chow or control chow for 3 months.
Tables and graphs zip file:
-Tabular data and graphs in Prism that were used to generate graphs shown in all figures except Fig 7DIJ. Note that the cilia length is in µm, and the area measurements by CellProfiler software in Figure 7 are in pixels.
-Tabular data in .csv format that was used to generate graphs shown in all figures except Fig 7DIJ. Note that the cilia length is in µm and the area measurements by CellProfiler software in Figure 7 are in pixels.
Fig 7 pipelines zip file:
CellProfiler software pipelines used for quantification in Figure 7BCEGHK and Figure 7I.
Raw values.zip:
Raw tabular data in.csv format from quantitation in all figures except Fig 7DIJ. The percentage of ciliation and the number of RNA dots were scored manually. Cilia length was measured using FIJI. Automated determination of density and intensity of dopaminergic processes in the striatum were done using CellProfiler. Note that the cilia length is in µm and the area measurements by CellProfiler software in Figure 7 are in pixels.
Key resource table file:
.csv format Key resource table describing the resources used in the study.
Sharing/Access information
Licenses/restrictions placed on the data: None Currently Applicable
Links to publications that cite or use the data: None Currently Applicable
Links to other publicly accessible locations of the data: None Currently Applicable
Links/relationships to ancillary data sets: None Currently Applicable
Was data derived from another source? No
Version change log
Version 1: August 8, 2024
Version 2: November 18, 2024
The following changes were added as part of the revision process.
1. Figure 7ABCD was newly added.
2. Figure 7ABCD in the previous version is now renamed Figure 7EFGH to reflect their new order.
3. Updated Raw figure files zip file to incorporate updated images of Figure 2D and newly added images from Fig 7A and Fig 7E (7A in the previous version).
4. Added new Raw Fig S1 zip file to incorporate newly added images in Fig S1.
5. Added Raw Fig S2 zip file to incorporate newly added images in Fig S2.
6. Added Raw Fig S3 zip file to incorporate newly added images in Fig S3.
7. Tables and graphs zip file updated to incorporate newly added data from Fig 7BCD, Fig S1, and Fig S4. Renamed data from Fig 7BCD in the previous version to Fig 7FGH to reflect their new order. All the files have now been updated in a tidy format.
8. Renamed Figure 7BCD pipeline zip file to Figure 7BCDFGH pipeline zip file to indicate that the same pipeline is used in all the analyses of Figure 7.
9. Added raw values from newly added Fig 7B-D and renamed previous Fig 7B-D as Fig 7F-H to reflect their new order in the Raw values zip file. All the files have now been updated in a tidy format.
10. Updated Key resource table to add RRID for total Rab12 antibody.
Version 3: February 26, 2025
The following changes were made in February 2025 to revise the manuscript.
1. Renamed Fig 7ABCD zip file to Fig 7ABCE to reflect their new order.
2. Renamed Fig 7EFGH zip file to Fig 7FGHIK to reflect their new order and added raw images for Fig 7FI.
3. Added new Fig 7DJ zip file to incorporate newly added data in Figure 7DJ.
4. Added new Fig 7I zip file to incorporate newly added data in Figure 7I.
5. Added new Raw Fig S4 zip file to incorporate newly added data in Figure S4.
6. Added new Raw Fig S5 zip file to incorporate newly added data in Figure S5.
7. Updated the key resource table to add antibodies that were used to revise the paper.
8. Renamed Fig7BCDFGH pipeline zip file to Fig 7 pipelines to indicate the cell profiler software pipelines used in Figure 7.
Version 4: March 28, 2025
The following changes were made in March 2025 to revise the manuscript.
- The new Figure 1HIJK zip file was added to incorporate new data as part of the revision.
Reagents
MLi-2 LRRK2 inhibitor was synthesized by Natalia Shpiro (MRC Reagents and Services, University of Dundee) and was first described to be a selective LRRK2 inhibitor in previous work (M.J Fell et al., 2015). For the MLi-2 in diet study, rodent diet containing MLi-2 at 360 mg per Kg was manufactured by Research diets, Inc.
Research standards for animal studies
Mice were maintained under specific pathogen-free conditions at the University of Dundee (UK). All animal experiments were ethically reviewed and conducted in compliance with the Animals (Scientific Procedures) Act 1986 and guidelines established by the University of Dundee and the U.K. Home Office. Ethical approval for animal studies and breeding was obtained from the University of Dundee ethical committee, and all procedures were performed under a U.K. Home Office project license. The mice were group-housed in an environment with controlled ambient temperature (20–24°C) and humidity (45–55%), following a 12-hour light/12-hour dark cycle, with ad libitum access to food and water. LRRK2 R1441C knock-in mice backcrossed on a C57BL/6J background, were obtained from the Jackson laboratory (Stock number: 009346). LRRK2 G2019S knock-in mice backcrossed on a C57BL/6J background, were obtained from Taconic (Model 13940). Genotyping of mice was performed by PCR using genomic DNA isolated from tail clips or ear biopsies with genotyping confirmation conducted on the day of the experiment.
In-diet MLi-2 administration
R1441C LRRK2 homozygous knock-in mice (8 weeks of age) were allowed to acclimate to the control rodent diet (Research Diets D01060501; Research Diets, Inc., New Brunswick, NJ) for 14 days before being placed on study at 10 weeks of age. On day 1 of the study, one group (9 mice) received a modified rodent diet targeted to provide a concentration of 60 mg/kg per day of MLi-2 on the basis of an average food intake of 5 g/day (D19012904); the other group (9 mice) received an untreated diet and served as the control group. Bodyweight and food intake were assessed twice weekly. On day 91, mice were culled and tissues collected as described below. For the brain analyses, 9 R1441C LRRK2 litter #1 mates were used (6 MLi-2-fed, 3 controls) plus 3 additional age-matched R1441C LRRK2 controls (from litter #2). For immunoblotting analysis, 6 additional R1441C LRRK2 mice from litter #2 were used: 3 control and 3 MLi-2-fed. For all other experiments, littermates were used.
Mouse brain processing
Mouse brains were fixed by transcardial perfusion using 4% paraformaldehyde (PFA) in PBS as described in dx.doi.org/10.17504/protocols.io.bnwimfce. Whole brain tissue was extracted, post-fixed in 4% PFA for 24 hr and then immersed in 30% (w/v) sucrose in PBS until the tissue settled to the bottom of the tube (~48 hr). The brains were harvested in Dundee and sent with identities blinded until analysis was completed. Prior to cryosectioning, brains were embedded in cubed-shaped plastic blocks with OCT (BioTek, USA) and stored at −80 °C. OCT blocks were allowed to reach −20 °C for ease of sectioning. The brains were oriented to cut coronal sections on a cryotome (Leica CM3050S, Germany) at 16–25 µm thickness and positioned onto SuperFrost plus tissue slides (Thermo Fisher, USA).
Mouse tissue processing for immunoblotting analysis
Three mice from each group (control diet and MLi-2 diet) were euthanized by cervical dislocation. Tissues including brain and kidney were collected and rinsed twice with cold PBS containing phosphatase and protease inhibitors (PhosSTOP, Merck #04906837001, and complete EDTA-free Protease Inhibitor Cocktail, Roche #11836170001) before being snap-frozen. Frozen mouse brains were placed on a stainless steel adult mouse brain slicer matrix (EMS #69090-C) kept on dry ice. Using this setup, 1.0-mm coronal sections were prepared. Landmarks in each slice were aligned with those in a reference atlas and the regions were excised using a cold scalpel or biopsy punch. For the immunoblotting shown in Fig. 1, an entire 1-mm coronal section from each sample was taken at approximately -2.3 mm rostrocaudal from bregma. The sections were then stored at -80 °C until further processing for immunoblotting analysis (https://doi.org/10.17504/protocols.io.bsgrnbv6).
Quantitative immunoblotting analysis
Tissue analysis by immunoblot to measure levels of Rab10, phospho-T73 Rab10, Rab12, phospho-S105 Rab12, LRRK2, and phospho-S935 LRRK2 was performed as described in https://doi.org/10.17504/protocols.io.bsgrnbv6. Briefly, snap-frozen tissues were thawed on ice in a tenfold volume excess of ice-cold lysis buffer containing 50 mM Tris–HCl pH 7.4, 1 mM EGTA, 10 mM 2-glycerophosphate, 50 mM sodium fluoride, 5 mM sodium pyrophosphate, 270 mM sucrose, supplemented with 1 μg/mL microcystin-LR, 1 mM sodium orthovanadate, cOmplete EDTA-free protease inhibitor cocktail (Roche), and 1% (v/v) Triton X-100 and homogenized using a Precellys Evolution system, employing three cycles of 20 s homogenization (6800 rpm) with 30 s intervals. Lysates were centrifuged at 15,000 × g for 30 min at 4°C, and supernatants were collected for subsequent Bradford protein assay and immunoblot analysis. The following primary antibodies were used: mouse anti-total LRRK2 (Neuromab N241A/34), rabbit anti-LRRK2 pS935 (UDD2 10(12), MRC Reagents and Services), rabbit anti-pT73 Rab10 (ab230261, Abcam), mouse anti-total Rab10 (0680–100/Rab10-605B11, Nanotools), rabbit anti-pS106 Rab12 (ab256487, Abcam), rabbit anti-total Rab12 (A26172, ABclonal). Primary antibody probes were detected using IRDye labeled secondary antibodies (IRDye 680LT Donkey anti-Mouse IgG; IRDye 800CW Donkey-anti-Rabbit IgG). Protein bands were acquired via near-infrared fluorescent detection using the Odyssey CLx imaging system and quantified using Image Studio Lite (Version 5.2.5, RRID:SCR_013715).
Immunoblotting of 25-30µm thick, fixed frozen mouse brain cryosections for tyrosine hydroxylase was performed following the protocol of Krebs et al. (28). Slides were brought from -80°C and placed on a cool platform. Sections were visualized under a dissection microscope, and all regions except the dorsolateral striatum were scraped off using a scalpel. Sections with dorsolateral striatum (roughly 3mm2 per hemisphere) were then lysed by adding 2% SDS, 0.05 M DTT, 10% glycerol, 1 mM EDTA, 60 mM Tris-HCl, pH 7.2 (a total volume of 15µl lysis buffer per section), heating at 95°C for 10 minutes, followed by centrifugation at 10,000 x g for 10 minutes at RT. The supernatant was analyzed by immunoblotting using sheep anti-tyrosine hydroxylase (AB1542, Millipore Sigma) and mouse anti-GAPDH (sc-32233, Santa Cruz Biotechnology) primary antibodies and DyLight™ 800 rabbit anti-sheep IgG (H+L) and IRDye 680RD Donkey anti-Mouse IgG secondary antibodies. Blots were imaged using the Odyssey Infrared scanner (LI-COR) and quantified using FIJI (RRID:SCR_002285).
Immunohistochemical staining
The mouse brain striatum was subjected to immunostaining following a previously established protocol (dx.doi.org/10.17504/protocols.io.bnwimfce). Frozen slides were thawed at room temperature for 15 minutes and then gently washed twice with PBS for 5 minutes each. Antigen retrieval was achieved by incubating the slides in 10 mM sodium citrate buffer pH 6.0, preheated to 95°C, for 15 minutes. Sections were permeabilized with 0.1% Triton X-100 in PBS at room temperature for 15 minutes, followed by blocking with 2% FBS and 1% BSA in PBS for 2 hours at room temperature. Primary antibodies were applied overnight at 4°C, and the next day, sections were exposed to secondary antibodies at room temperature for 2 hours. Secondary antibodies used were donkey highly cross-absorbed H + L antibodies conjugated to Alexa 488, Alexa 568, or Alexa 647, diluted at 1:2000. Nuclei were counterstained with 0.1 μg/ml DAPI (Sigma). Finally, stained tissues were mounted with Fluoromount G and covered with a glass coverslip. All antibody dilutions for tissue staining contained 1% DMSO to facilitate antibody penetration. Automated determination of the density and intensity of dopaminergic processes in the striatum was carried out as described: dx.doi.org/10.17504/protocols.io.x54v92km4l3e/v1.
Fluorescence in situ hybridization (FISH)
RNAscope fluorescence in situ hybridization was carried out as described (https://bio-protocol.org/exchange/protocoldetail?id=1423&type=3). The RNAscope Multiplex Fluorescent Detection Kit v2 (Advanced Cell Diagnostics) was utilized following the manufacturer's instructions, employing RNAscope 3- plex Negative Control Probe (#320871) or Mm-Ptch1-C2 (#402811-C2), Mm-Gdnf (#421951), Mm-Nrtn-C2 (#441501-C2), Mm-Pvalb-C3 (#421931-C3) and Mm-Shh-C2 (#314361-C2). The Mm-Ptch1-C2, Mm-GDNF, Mm-Pvalb-C3, and Mm-Nrtn-C2 probes were diluted 1:5, 1:20, 1:10, and 1:3, respectively in dilution buffer consisting of 6x saline-sodium citrate buffer (SSC), 0.2% lithium dodecylsulfate, and 20% Calbiochem OmniPur Formamide. Fluorescent visualization of hybridized probes was achieved using Opal 690 or Opal 570 (Akoya Biosciences). Subsequently, brain slices were subjected to blocking with 1% BSA and 2% FBS in TBS (Tris buffered saline) with 0.1% Triton X-100 for 30 minutes. They were then exposed to primary antibodies overnight at 4°C in TBS supplemented with 1% BSA and 1% DMSO. Secondary antibody treatment followed, diluted in TBS with 1% BSA and 1% DMSO containing 0.1 μg/ml DAPI (Sigma) for 2 hours at room temperature. Finally, sections were mounted with Fluoromount G and covered with glass coverslips.
Microscope image acquisition
All images were obtained using a Zeiss LSM 900 confocal microscope (Axio Observer Z1/7) coupled with an Axiocam 705 camera and immersion objective (Plan-Apochromat 63x/1.4 Oil DIC M27). The images were acquired using ZEN 3.4 (blue edition) software, and visualizations and analyses were performed using Fiji and CellProfiler.
In addition to above-mentioned methods, all other statistical analysis was carried out using GraphPad Prism version 10.2.3 for Macintosh, GraphPad Software, Boston, Massachusetts USA, www.graphpad.com.