Data from: Polybrominated diphenyl ether (DE-71) exposure skews phenotypic sex ratio, and alters steroid hormone levels and steroidogenic enzyme activities in juvenile Silurana tropicalis
Fort, Douglas J.
Fort, Troy D.
Guiney, Patrick D.
Weeks, John A.
Published Jul 26, 2019 on Dryad.
Cite this dataset
Fort, Douglas J. et al. (2019). Data from: Polybrominated diphenyl ether (DE-71) exposure skews phenotypic sex ratio, and alters steroid hormone levels and steroidogenic enzyme activities in juvenile Silurana tropicalis [Dataset]. Dryad. https://doi.org/10.5061/dryad.q4qd540
The impact of the brominated flame-retardant mixture, DE-71, on gonadal steroidogenesis during sexual differentiation in Silurana tropicalis was examined. A partial lifecycle study exposing S. tropicalis to varying concentrations of DE-71 (0.0, 0.65, 1.3, 2.5, and 5.0 g/L [nominal]) was conducted from early gastrula stage embryo to 150 d post-metamorphosis (dpm). Exposure of S. tropicalis to DE-71 induced liver necrosis and induced abnormal ovary development characterized by previtellogenic oocyte necrosis and arrested development of vitellogenic oocytes in females in a concentration-dependent manner. Decreased mean plasma DHT and T, gonad T, and increased mean plasma E2 levels were found in 150 dpm DE-71-treated male S. tropicalis compared to controls. Plasma E2 levels in females were not significantly altered compared to control S. tropicalis, although lower plasma and gonad T were detected. Mean gonadal CYP 19 aromatase activity in both male and female S. tropicalis exposed to DE-71 was not appreciably affected. Decreased mean male 5α-reductase and CYP17 activities in both male and females were observed compared to control frogs. Overall, these studies suggested that PBDE exposure induced liver necrosis and abnormal ovary development; and reduced circulating and gonadal androgens resulting in a phenotypic skew in sex ratio toward the female sex in S. tropicalis