Data from: Restoration of pyrethroid susceptibility in a highly resistant Aedes aegypti population

Grossman, Marissa K.1; Uc-Puc, Valentin2; Rodriguez, Julian2; Culter, David J.2; Morran, Levi T.2; Manrique-Saide, Pablo3; Vazquez-Prokopec, Gonzalo M.2; Cutler, David J.2

Published May 23, 2018 on Dryad. https://doi.org/10.5061/dryad.t2n49

Cite this dataset

Grossman, Marissa K. et al. (2018). Data from: Restoration of pyrethroid susceptibility in a highly resistant Aedes aegypti population [Dataset]. Dryad. https://doi.org/10.5061/dryad.t2n49

Abstract

Insecticide resistance has evolved in disease vectors worldwide, creating the urgent need to either develop new control methods or restore insecticide susceptibility to regain the use of existing tools. Here we show that phenotypic susceptibility can be restored in a highly resistant field-derived strain of Aedes aegypti in only ten generations through rearing them in the absence of insecticide.

Usage notes

Allele-specific PCR

Allele-specific PCR for two kdr mutations. The two numbers under the 1534 and 1016 columns indicate the two alleles: For 1534, 83 is the melting point for the resistant allele and 79 is susceptible; for 1016, 83 is susceptible and 79 is resistant.

PCR_tent_data.xlsx

CDC bottle bioassay data

Data from CDC bottle bioassays at F10 using both permethrin and deltamethrin. Time is in minutes. KD = "knock-down."

BBA_tents.xlsx

Funding

National Science Foundation, Award: 1601520