Data from: Isolation of diverse simian arteriviruses causing hemorrhagic disease
Data files
Jul 11, 2024 version files 62.69 KB
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DRYAD_upload_for_simian_arterivirus_isolation.xlsx
61.35 KB
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README.md
1.34 KB
Abstract
Genetically diverse simian arteriviruses (simarteriviruses) naturally infect geographically and phylogenetically diverse monkeys, and cross-species transmission and emergence are of considerable concern. Characterization of most simarteriviruses beyond sequence analysis has not been possible because the viruses fail to propagate in the laboratory. We attempted to isolate 4 simarteriviruses, Kibale red colobus virus 1, Pebjah virus, simian hemorrhagic fever virus, and Southwest baboon virus 1, by inoculating an immortalized grivet cell line (known to replicate simian hemorrhagic fever virus), primary macaque cells, macrophages derived from macaque induced pluripotent stem cells, and mice engrafted with macaque CD34+-enriched hematopoietic stem cells. The combined effort resulted in successful virus isolation; however, no single approach was successful for all 4 simarteriviruses. We describe several approaches that might be used to isolate additional simarteriviruses for phenotypic characterization. Our results will expedite laboratory studies of simarteriviruses to elucidate virus-host interactions, assess zoonotic risk, and develop medical countermeasures.
https://doi.org/10.5061/dryad.t4b8gtj5r
Description of the data and file structure
Each tab in the .csv file corresponds to the data from a figure in the corresponding manuscript. The data provided in this dataset are raw Ct values derived from RT-qPCR performed with virus-specific primers. DPI= days post infection. All blank cells are left intentionally blank.
Figure 1: shows Ct values generated from RT-qPCR, for 4 different viral isolates inoculated onto MA-104 cells, 3 replicates per virus.
Figure 4&5: shows Ct values generated from RT-qPCR, for 4 different viral isolates inoculated onto 4 different types of tissues obtained from rhesus macaque (adherent PBMC, non-adherent PBMC, adherent splenocytes, non-adherent splenocytes), at mutiple DPI. For viruses SHFV, KRCV-1, and SWBV, this was performed in duplicate. For PBJV, this was performed in triplicate.
Figure 8: shows Ct values generated from RT-qPCR, for 4 different viral isolates inoculated onto iPSC-derived macaque macrophages. Variable DPI and number of replicates depending on virus.
Figure 9: Shows details regarding animal infection studies including mouse background, engraftment status, virus, and DPI. Each row is a single animal.