Previous models of duplication-based new gene evolution have overlooked the role of genome architecture. The enhancer-capture divergence model provides a one-step mechanism for altering the expression of a pre-existing protein. By duplicating into a region of the genome that is under the influence of a pre-existing enhancer, distal duplication of an existing gene provides for immediate augmentation of spatiotemporal expression of the existing gene. In the related manuscript, we examine the local chromosomal conformations for a newly evolved gene HP6/Umbrea, which appears to have undergone the enhacer-capture divergence process. While all data from the associated manuscript are publicly available through permanent NCBI repositories, additional related 4C-Seq data was generated but did not appear in the final manuscript. The following data set consists of 1) raw reads from 4C-Seq experiments performed on D. melanogaster, D. yakuba, D. erecta, and D. pseudoobscura for HP6/Umbrea and a neighboring locus, 2) barcodes for de-multiplexing the reads, and 3) code for running analyses from the related manuscript.

Imaginal disc tissue from 4 species was extracted from L3 wandering larvae. A standard 4C-Seq protocol was performed with a primary digest of DpnII and a secondary digest of Csp6i. Further details may be found in the associated manuscript.