Skip to main content
Dryad

Differentially-expressed genes in blood in response to lipopolysaccharide in three rodent species

Abstract

Infection tolerance in rodents was examined by injecting single-dose lipopolysaccharide (LPS) to induce inflammation in Peromyscus leucopus (LL stock), the white-footed deermouse also reservoir for Lyme disease and Mus musculus (outbred CD-1 breed), the house mouse, and Rattus norvegicus, the brown rat (Fischer strain). Reaction to LPS was analyzed in the blood of challenged rodents and compared to control animals. As natural reservoirs of zoonoses deermice show significant anti-inflammatory response as described in "An Infection-Tolerant Mammalian Reservoir for Several Zoonotic Agents Broadly Counters the Inflammatory Effects of Endotoxin" (https://doi.org/10.1128/mBio.00588-21). The project and the description of the samples are described under the following NCBI BioProjects: PRJNA975149 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA975149) for mouse and deermouse and PRJNA973677 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA973677). This project is a follow-up project focusing on the transcriptomic analysis of the whole blood bulk RNA-seq and further analysis of differentially expressed genes (DEG) between the treatment arm and controls. Complete fold change and false discovery rate for all three rodent species used for the current Dryad set are previously published (https://doi.org/10.7280/D1470Z). Here we report that deermice tolerance to infection is partly due to lower expression of interferon-gamma in comparison to mice and rats.