"Source data for Figure 2B: Invagination of the membrane for 8 embryos (delta x), mean (mean delta) and adjusted mean in fixed tissues (mean delta fixed) as function of time during n.c. 14. NaN indicates that furrow canal could not be detected at that particular time." Time delta 1 delta 2 delta 3 delta 4 delta 5 delta 6 delta 7 delta 8 mean delta mean delta fixed (min) (um) (um) (um) (um) (um) (um) (um) (um) (um) (um) 0.875 NaN 1.9813 NaN NaN NaN 0.61509 NaN NaN 1.2982 1.2333 1.875 NaN 1.7186 NaN 0 NaN 1.6089 0.57818 0.087701 0.79441 0.75469 2.875 NaN 2.0496 NaN 0.4255 0.57507 1.7429 0.71157 0.16171 0.9444 0.89718 3.875 NaN 2.847 NaN 1.2428 0.6894 1.6089 1.2051 0.21922 1.3021 1.237 4.875 1.0189 2.1853 NaN 0.70864 1.412 1.6751 2.3089 0.20021 1.3584 1.2905 5.875 1.1671 2.9275 1.9523 0.91533 1.6712 1.8071 2.3783 1.2297 1.7561 1.6683 6.875 1.7631 2.9275 2.4686 1.2428 2.0402 1.8754 2.64 1.9442 2.1127 2.0071 7.875 1.4634 3.4503 2.3853 2.0256 2.1501 2.2714 1.7514 2.2199 2.2147 2.1039 8.875 2.3553 3.0455 3.4991 2.3111 2.4717 2.7365 2.3414 2.3476 2.6385 2.5066 9.875 2.0561 2.5161 3.3578 2.0151 2.5868 2.2714 2.3622 2.9378 2.5129 2.3873 10.875 2.7995 2.9089 2.7979 2.5445 2.8893 2.4075 2.5098 3.0092 2.7333 2.5966 11.875 2.7995 2.5776 2.6595 2.7439 2.6685 2.869 2.7156 2.8075 2.7301 2.5936 12.875 2.5033 2.9751 3.1275 2.8146 2.6805 2.9401 3.3957 3.7447 3.0227 2.8716 13.875 2.5115 3.1071 3.7345 3.2754 2.878 3.2653 2.4918 3.2114 3.0594 2.9064 14.875 3.5378 3.3957 3.9676 3.6066 3.2969 3.795 3.2616 3.671 3.5665 3.3882 15.875 3.8423 3.5714 3.8739 3.6066 3.8377 3.8782 2.7156 3.7447 3.6338 3.4521 16.875 3.5378 3.9753 3.6503 3.6783 3.5064 3.6629 3.6912 4.2782 3.7475 3.5602 17.875 3.3896 3.969 3.1275 4.0027 3.9343 3.3989 3.675 4.333 3.7288 3.5423 18.875 3.8423 3.3064 3.4991 4.1197 3.8603 3.9942 3.9868 3.9478 3.8196 3.6286 19.875 4.2805 3.9027 4.0682 4.6539 3.9343 3.5966 4.8361 4.333 4.2007 3.9906 20.875 3.8362 4.171 4.3423 4.2114 4.1605 4.5905 4.1262 4.3942 4.229 4.0176 21.875 4.873 3.9753 4.3935 4.3401 4.5539 4.0614 4.5656 4.9952 4.4697 4.2463 22.875 5.4656 4.5786 4.1541 4.1386 4.3935 4.5905 4.9145 4.2627 4.5623 4.3341 23.875 5.0464 4.6989 4.5776 4.334 4.8822 4.0614 5.5212 4.5939 4.7145 4.4787 24.875 4.8782 5.1644 5.0333 5.002 4.5618 5.1877 5.3404 5.2699 5.0547 4.802 25.875 4.7302 5.1038 5.046 4.9333 4.9729 5.0683 5.8975 4.1055 4.9822 4.7331 26.875 5.0262 4.8966 5.4688 5.7943 4.7862 4.9218 5.9305 5.3263 5.2688 5.0054 27.875 5.0195 5.4346 5.2793 5.4346 5.2249 5.3865 6.004 6.9128 5.587 5.3077 28.875 5.7618 5.9013 6.0283 4.8729 5.3919 6.322 5.6354 6.1191 5.7541 5.4664 29.875 5.4656 6.2322 6.5958 5.9392 5.8496 6.4517 6.342 6.382 6.1573 5.8494 30.875 6.2063 6.5574 6.3564 6.4567 6.3609 6.7223 7.2356 6.1896 6.5106 6.1851 31.875 6.5135 6.5531 7.2471 6.5275 6.6491 6.9178 7.3747 6.5815 6.7955 6.4558 32.875 6.7975 7.547 7.482 6.3983 6.5552 6.9759 7.5671 6.9784 7.0377 6.6858 33.875 7.8394 7.953 8.0904 8.641 7.5027 6.6446 8.2775 7.6443 7.8241 7.4329 34.875 7.9829 7.9482 7.3424 8.6443 8.5348 7.189 9.0705 8.3205 8.1291 7.7226 35.875 8.4285 8.375 8.33 10.005 9.3729 8.1022 9.1658 7.9897 8.7211 8.2851 36.875 8.5755 10.065 8.7477 9.8385 10.002 9.4458 10.216 9.7016 9.574 9.0953 37.875 10.066 10.795 9.9786 10.629 9.8983 9.6291 10.681 10.234 10.239 9.7269 38.875 10.946 11.394 10.585 11.581 12.097 9.5602 9.5734 11.267 10.875 10.332 39.875 12.132 12.477 12.267 12.157 12.437 10.356 12.196 11.831 11.982 11.383 40.875 12.727 13.258 13.612 13.351 13.251 12.229 13.609 11.573 12.951 12.304 41.875 13.465 14.173 14.181 14.076 14.975 14.41 14.492 13.376 14.144 13.436 42.875 14.058 14.836 14.609 15.077 15.476 13.552 14.704 15.332 14.705 13.97 43.875 15.391 15.048 16.104 15.882 16.424 15.662 14.701 16.151 15.67 14.887 44.875 15.985 16.765 17.274 16.07 16.744 16.388 16.166 16.657 16.506 15.681 45.875 16.873 16.855 18.211 17.885 17.966 17.461 16.465 18.322 17.505 16.63 46.875 17.913 18.69 18.961 18.251 18.615 17.721 19.136 18.914 18.525 17.599 47.875 18.8 19.74 20.321 20.045 19.863 18.882 20.893 21.095 19.955 18.957 48.875 20.43 20.606 21.775 20.37 20.457 20.774 20.66 22.006 20.885 19.84 49.875 21.915 21.641 22.522 22.163 22.393 21.491 22.74 23.23 22.262 21.149 50.875 23.393 24.777 24.724 23.751 24.695 24.602 23.863 24.457 24.283 23.069 51.875 25.32 25.865 26.07 25.407 25.636 25.333 25.66 26.823 25.764 24.476 52.875 27.098 28.487 28.754 27.714 27.618 26.898 28.274 27.665 27.814 26.423 53.875 28.876 29.793 30.909 29.14 29.986 28.646 31.022 30.507 29.86 28.367 54.875 30.362 32.07 32.196 30.777 30.457 30.378 35.511 31.297 31.631 30.049 55.875 32.137 34.215 34.427 NaN 31.593 30.614 35.542 33.353 33.126 31.47 56.875 34.955 35.89 35.455 NaN 32.543 33.712 36.472 34.414 34.777 33.038 57.875 35.987 37.269 NaN NaN 35.123 35.171 NaN 37.459 36.202 34.392 58.875 35.988 37.651 NaN NaN 37.39 36.618 NaN 38.795 37.289 35.424 59.875 37.618 36.311 NaN NaN NaN 38.421 NaN 38.188 37.634 35.753 60.875 39.395 NaN NaN NaN NaN NaN NaN NaN 39.395 37.425 61.875 37.765 NaN NaN NaN NaN NaN NaN NaN 37.765 35.877