Title: Impacts of soil nitrogen and phosphorus levels on cytotype performance of the circumboreal herb, Chamerion angustifolium: implications for polyploid establishment Authors: Angela M. Walczyk (amwalczy@mtu.edu), Erika I. Hersch-Green (eherschg@mtu.edu) Year: 2018 *Analyses preformed in JMP Pro 13.0 software package (SAS Institute, Cary, North Carolina, USA): https://www.jmp.com/en_gb/software/predictive-analytics-software.html ----------------------------------------- Datafile: Walczyk & Hersch-Green 2019_AJB_Dryad File_1 Below outlines a description of each data file including data types, correspondence to tables/figures, and identification of variable and header coding. The following column labels are consistant throughout all data files when present... Plant: Identification of each experimental plant. Letters were used to differentiate plants grown in the same pot. Nut: Nutrient. Either low or high nitrogen:phosphorus treatment Comp: Competition. The type of competition a plant was exposed to (alone, inter-cytotype, intra-cytotype) Comp 2: Competition 2. Whether a plant was grown alone or in competition. Focal Pldy: Focal plant's ploidy level. (2x = diploid, 4x=established tetraploid, 4x' = neotetraploid) Comp Pldy: Ploidy level of a plant's competitor. Plants grown alone have "alone" in this column. Geno: Maternal genetic line of a focal plant. Survive: Whether a plant survived until the end of the experiment (1= alive, 0=dead). Cage: if the plant was used in the whole-plant insect bioassay (1=yes, 0=no) Dam all plants: The numerical amount of damage inflicted upon a plant by Spodoptera exigua during the whole plant cage assays. Plants not included in the whole plant choice bioassay are included in this column to use as covariates in MANCOVA models. Cage % Damage: The numerical amount of damage inflicted upon a plant by Spodoptera exigua during the whole plant cage assays. Cells with no values indicate a plant that was not included in the assay. This column was used as a main response variable in ANCOVA models to evaluate insect feeding damage. Height (#/#): The height (cm) of each focal plant at a corresponding date (i.e. 3/15/2018) during the experiment. Phen.: Phenology of plant at harvest/final heigh measurement on an ordinal scale. 1= basal rosette, 2= elongated stem (no flower buds at all), 3= immature flower buds (small and green color), 4= mature flower buds (pink color), 5= at least one mature flower, 6= more than one mature fruit Final Height: Height measured at harvest. Stem width: Width in centimeters of stem just above soil line at harvest. Original Thrip Total: Ordinal scale of F. occidentalis damage for the all plant foliage at harvest:0 = none, 1 = 1-5%, 2 = 6-12%, 3 = 12-20%, 4 = 21-40%, 5= 51-60%, 6 = 61-70%, 7 = +70% Flowering: if a plant produced at least one mature flower (1= yes, 0=no) Days to flower: The number of days since germination that it took a plant to produce its first flower. This value was square root transformed in the "Sqrt Days to Flower" column. Flower Duration: The number of days a plant flowered for. This value was square root transformed in the "Sqrt Flower duration" column. # flowers: the number of mature flowers a plant produced during its life. This value was square root transformed in the "Sqrt # Flowers" column Flower Thrip: Ordinal scale score flower damage severity caused by F. occidentalis, 0= none, 1=mild (pollen eaten, no damage to anther/style), 2=moderate (anthers damaged), 3=severe (anthers and style damage, flower sterile). Above (g): Dried aboveground biomass. This value was square root transformed in the "Sqrt above (g)" column Below (g): Dried belowground biomass. This value was square root transformed in the "Sqrt below (g)" column Total mass (g): Combined aboveground and belowground plant mass. This value was square root transformed in the "Sqrt total mass" column. # Root buds: The number of root buds produced by a plant at harvest. This value was square root transformed in the "Sqrt # root bud column" 1. -------------------- Statistical Test: Binomial logistic Regression for survival likelihood File name: Walczyk & Hersch-Green 2019_AJB_Dryad File_1 Notes: All plants were included in this test. Model Factor(s): Focal Pldy Model Response(s): Survive 2. -------------------- Statistical Test: 3-way binomial logistic Regression for flowering likelihood File name: Walczyk & Hersch-Green 2019_AJB_Dryad File_1 Notes: Plants that died during the experiment were excluded in this test. Plant 12, 51, and 79 were found to be triploid and were excluded from all analyses. Corresponding Tables/Figures: Table 1, Appendix S2 Model Factor(s): Focal Pldy, Comp2, Nut, Focal Pldy x Comp2, Focal Pldy x Nut, Comp2 x Nut, Focal Pldy x Comp2 x Nut Model Response(s): Flowering 4.-------------------- Statistical Test: ANCOVA for flower production File name: Walczyk & Hersch-Green 2019_AJB_Dryad File_1 Notes: Plants that died during the experiment were excluded in this test. Plants that did not flower were not included in this test. Plant 12, 51, and 79 were found to be triploid and were excluded from all analyses. Corresponding Tables/Figures: Table 2 Model Factor(s): Focal Pldy, Comp Pldy, Nut, Focal Pldy x Comp Pldy, Focal Pldy x Nut, Comp Pldy x Nut, Focal Pldy x Comp Pldy x Nut Covariate(s): Ordinal Thrip Total Model Response(s): Sqrt # Flowers 5.-------------------- Statistical Test: 3-way MANCOVA for final height, aboveground biomass, belowground biomass, and root buds File name: Walczyk & Hersch-Green 2019_AJB_Dryad File_1 Notes: Plants that died during the experiment were excluded in this test. Plant 12, 51, and 79 were found to be triploid and were excluded from all analyses. Corresponding Tables/Figures: Appendix S3 Model Factor(s): Focal Pldy, Comp Pldy, Nut, Focal Pldy x Comp Pldy, Focal Pldy x Nut, Comp Pldy x Nut, Focal Pldy x Comp Pldy x Nut Covariate(s): Ordinal Thrip Total, Dam all Plants Model Response(s): Final height, Sqrt above (G), sqrt below (g), sort # Rootbuds 6. -------------------- Statistical Test: 2-way MANCOVA for final height, aboveground biomass, belowground biomass, and root buds by nutrient treatment File name: Walczyk & Hersch-Green 2019_AJB_Dryad File_1 Notes: Plants that died during the experiment were excluded in this test. Plant 12, 51, and 79 were found to be triploid and were excluded from all analyses. Corresponding Tables/Figures: Table 3, Fig 1, Fig 2 Model Factor(s): Focal Pldy, Comp Pldy, Focal Pldy x Comp Pldy, by Nut Covariate(s): Ordinal Thrip Total, Dam all Plants Model Response(s): Final height, Sqrt above (G), sqrt below (g), sort # Rootbuds 7.-------------------- Statistical Test: Ordinal logistic Regression for foliar damage from F. occidentalis File name: Walczyk & Hersch-Green 2019_AJB_Dryad File_1 Notes: Plants that died during the experiment were excluded in this test. Plant 12, 51, and 79 were found to be triploid and were excluded from all analyses. Corresponding Tables/Figures: Table 5, Appendix S4 Model Factor(s): Focal Pldy, Comp 2, Nut, Focal Pldy x Comp 2, Focal Pldy x Nut, Comp 2 x Nut, Focal Pldy x Comp 2 x Nut Covariate: Final Height Model Response(s): Ordinal Thrip Total -------------------------------------------------------------