This file explains all of the files that accompany: Clark et al. “A whole methylomic study ethanol exposure in brain and blood: An exploration of the utility of peripheral blood as proxy tissue for brain in alcohol methylation studies” File: phenotype.txt -Contains all information used in associations testing; tab-delimited format - Variables (in order they appear in file): Sample_ID - identification number of the sample alcohol - indication of whether sample received acute Ethanol injection; 1= received ethanol, 0 = received saline Round - indication of which round of testing the mice came from; 1= Round 1; 2 = Round 2 Wildfire - indication of what software the sequencer was used at time sample was sequenced; 1= used Wildfire; 0 = did not use Wildfire Brain - indicator of whether sample is a brain or blood sample; 1= brain sample, 0 = blood sample File: Coverage.zip Compressed files that contains three files: Coverage.bmat -Filematrix with CpG Scores for all samples and all CpGs that passed the quality control steps outlined in the main manuscript -Each row has the CpG score for a single sample - Each column has the CpG score for a single CpG -See filematrix package for more format details (https://cran.r-project.org/web/packages/filematrix/index.html) Coverage.desc.txt - Text file containing information for R file matrix object Coverage.bmat - Contains information about the number of rows, number of columns, how information is stored, and size it is stored as Coverage.nmsrow.txt - Text file containing the row names for the R filematrix object Coverage.bmat - The row names are the Sample_ID of each sample in the order that they appear in the Coverage.bmat file File: CpG_locations.zip Compressed files that contains three files: CpG_locations.bmat Filematrix with the location of CpGs that passed quality control outlined in main manuscript. This file has two columns: chr and position. chr = chromosome, position = base pair position on mm10 See filematrix package for more format details (https://cran.r-project.org/web/packages/filematrix/index.html) CpG_locations.desc.txt - Text file containing information for R file matrix object CpG_locations.bmat - Contains information about the number of rows, number of columns, how information is stored, and size it is stored as CpG_locations.nmscol.txt - Text file containing the column names for the R filematrix object CpG_locations.bmat File: CpG_chromosome_names.txt - file with chromosome names for the integer column “chr” in CpG_locations* filematrix Each row has coverage for a single sample Each column has coverage for a single CpG Note: All file listed in this data packet are required for the analysis of the data in the R Bioconductor Package RaMWAS. Details of RaMWAS can be found here: https://bioconductor.org/packages/release/bioc/html/ramwas.html