This is the raw data for the manuscript "Ma et al., 2022. Traumatic mating causes strict monandry in a wolf spider" submitted to Proceedings of the Royal Society B: Biological Sciences. The dataset contains four Excel files: Artificial wounds.xlsx Column name: Description: Male No. Male number Female No. Female number Treatment (SF:Seminal fluid group; GTP: genital tract penetration group; GTPSF: genital tract penetration + seminal fluid group) Male body length(mm) Male body length Male body weight(g) Male body weight Male age(d) Male age after maturity Male carapace width(mm) Male carapace width Female body length(mm) Female body length Female body weight (g) Female body weight Female age (d) Female age after maturity Female carapace width(mm) Female carapace width Mating or not Female has mated or not Mating duration (s) Mating duration When mating occur When mating occurred after females were staged to mating trials. Embolus length.xlsx The embolus length of randomly chosen 20 males ( lengths of the pair of embolus were averaged for each individual) Free amino acid test.xlsx The concentration of each of the 20 free amino acid was tested for seminal fluids, mating plug and female haemolymph (3replicates). Longevity.xlsx The longevity of females that were inserted two copulatory openings, one copulatory opening, or umated. (20 replicates)