This readme.txt file was generated on 2022-09-22 by K. Skogen and E. Lewis   GENERAL INFORMATION   1. Title of Dataset: Oenothera Section Calylophus population genetic study   2. Author Information             A. Principal Investigator Contact Information                         Name: Krissa Skogen                         Institution: Clemson University                         Address: 132 Long Hall Clemson, SC 29631 USA                         Email: kskogen@clemson.edu               B. Associate or Co-investigator Contact Information                         Name: Emily Lewis                         Institution: Washington State University                         Address: 103 Neill Hall Pullman, WA 99163-3113 USA                         Email: emily.m.lewis@wsu.edu               C. Associate or Co-investigator Contact Information                         Name: Jeremie Fant                         Institution: Chicago Botanic Garden                         Address: 1000 Lake Cook Road Glencoe, IL, 60022 USA                         Email: jfant@chicagobotanic.org               D. Associate or Co-investigator Contact Information                         Name: Michael Moore                         Institution: Oberlin College                         Address: Science Center K111 Oberlin, OH 44074 USA                         Email: mmoore@oberlin.edu   3. Date of data collection (single date, range, approximate date) 2014 - 2015   4. Geographic location of data collection: New Mexico and Texas, USA   5. Information about funding sources that supported the collection of the data: NSF DEB 1342873     SHARING/ACCESS INFORMATION   1. Licenses/restrictions placed on the data: None   2. Links to publications that cite or use the data: Citation provided upon acceptance for publication   3. Links to other publicly accessible locations of the data: None   4. Links/relationships to ancillary data sets: None   5. Was data derived from another source? No   6. Recommended citation for this dataset: Citation provided upon acceptance for publication     DATA & FILE OVERVIEW   1. File List:   -FloralMorphGC_noYA_2021.csv: Floral morphology measurements for 333 growth-chamber grown O. hartwegii subsp. filifolia and O. gayleana plants, including corolla diameter, floral flare, etc.   -FloralMorphGC_noYA_2022.csv: Floral morphology measurements for 333 growth-chamber grown O. hartwegii subsp. filifolia and O. gayleana plants, including corolla diameter, floral flare, etc., with herkogamy left as blank if there was no style length measurement recorded.   -FST_GeogDistance.csv: For each pair of study sites, geographic distance (km), calculated FST, and linearized FST.   -GC_Crosses_December_2021.csv: The total seeds produced by each cross performed, with information on maternal population and cross type.   -GCWild_FloralMorphologyMaster.csv: Floral morphology measurements for 404 wild plants taken at in wild populations and 199 growth chamber plants grown from seeds collected at these wild populations.   -GeneticAnalysis_Chloro.csv: Population genetic parameters for the chloroplast haplotype analysis.   -GeneticAnalysis.csv: Population genetic parameters for the nuclear microsatellite genotype analysis.   -Matrix_Distance.csv: Matrix of pairwise geographic distances (km) between each of the populations.   -Matrix_FSTFili_Lin.csv: Matrix of pairwise linearized FST values between each of the O. hartwegii subsp. filifolia populations.   -Matrix_FstGay_Lin.csv: Matrix of pairwise linearized FST values between each of the O. gayleana populations.   2. Relationship between files, if important:   3. Additional related data collected that was not included in the current data package: None   4. Are there multiple versions of the dataset? FloralMorphGC_noYA_2021 and FloralMorphGC_noYA_2022 are very similar datasets, with only one column having different values. For ease of statistical computing, the 2022 version was saved as a new document.     METHODOLOGICAL INFORMATION   1. Description of methods used for collection/generation of data: Details of data collection are in the Methods section of the manuscript (citation provided upon acceptance for publication)   2. Methods for processing the data: Raw data files were processed in R scripts provided   3. Instrument- or software-specific information needed to interpret the data: NA   4. Standards and calibration information, if appropriate: NA   5. Environmental/experimental conditions: See details provided in (citation provided upon acceptance for publication)   6. Describe any quality-assurance procedures performed on the data: all data were entered and verified to correct data entry errors   7. People involved with sample collection, processing, analysis and/or submission: E. M. Lewis, K. A. Skogen, J. Fant, and M. Moore.     DATA-SPECIFIC INFORMATION FOR: FloralMorphGC_noYA_2021.csv: 1. Number of variables: 20 2. Number of cases/rows: 334 3. Variable List: Species: G = O. gayleana, F = O. hartwegii subsp. filifolia Population & Site: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D Individual: individual plant ID number MatLine: Maternal line ID number Flower: flower measured, 1 = first, 2 = second, 3 = third, 4 - fourth Date: Date data were collected corolla_diameter_1: corolla diameter (mm) measurement perpendicular to corolla_diameter_2 (mm) corolla_diameter_2: corolla diameter (mm) measurement perpendicular to corolla_diameter_1 Floral_flare: diameter (mm) of the opening of the floral tube Filament_length1: length of first filament (mm) Filament_length2: length of second filament (mm) Style_length: length from floral flare to end of style (mm) Tube_length: length from floral flare to insertion at ovary (mm) H: Simplified negative, neutral, or positive herkogamy Herkogamy: the physical separation between anthers and the stigma, was measured as the distance between the tallest anther and the stigma (mm) Notes: Notes taken during data collection Location: Location of plants included, GC = growth chamber PCA_Herk: Notation for R computations, yes = has style and filament measurement and can be used for herkogamy; no = missing one or more measurements.   DATA-SPECIFIC INFORMATION FOR: FloralMorphGC_noYA_2022.csv: 1. Number of variables: 20 2. Number of cases/rows: 334 3. Variable List: Species: G = O. gayleana, F = O. hartwegii subsp. filifolia Population: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Individual: individual plant ID number MatLine: Maternal line ID number Flower: flower measured, 1 = first, 2 = second, 3 = third, 4 - fourth Date: Date data were collected corolla_diameter_1: corolla diameter (mm) measurement perpendicular to corolla_diameter_2 (mm) corolla_diameter_2: corolla diameter (mm) measurement perpendicular to corolla_diameter_1 Floral_flare: diameter (mm) of the opening of the floral tube Filament_length1: length of first filament (mm) Filament_length2: length of second filament (mm) Style_length: length from floral flare to end of style (mm) Tube_length: length from floral flare to insertion at ovary (mm) H: Simplified negative, neutral, or positive herkogamy Herkogamy: the physical separation between anthers and the stigma, was measured as the distance between the tallest anther and the stigma (mm) Notes: Notes taken during data collection Location: Location of plants included, GC = growth chamber PCA_Herk: Notation for R computations, yes = has style and filament measurement and can be used for herkogamy; no = missing one or more measurements.   DATA-SPECIFIC INFORMATION FOR: FST_GeogDistance.csv: 1. Number of variables: 6 2. Number of cases/rows: 57 3. Variable List: Pop_pair: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Distance: distance between populations (km) Species: G = O. gayleana, F = O. hartwegii subsp. filifolia FST: FST as calculated by GenAlEx FST_Lin_Spag: FST linearized by Spaghedi program FST_Lin: FST linearized normally   DATA-SPECIFIC INFORMATION FOR: GC_Crosses_December_2021.csv: 1. Number of variables: 11 2. Number of cases/rows: 873 3. Variable List: CrossID: A unique number given to each cross Species: G = O. gayleana, F = O. hartwegii subsp. filifolia MaternalPop: Wild population the plant was descended from MaternalLine: Maternal line the plant was descended from (G = species, YA = maternal population, 3 = third maternal line from G_YA, 1 = first plant from G_YA_3, for G_YA_3_1) Maternal: Maternal plant (pollen recipient) Paternal: Paternal plant (pollen donor) PaternalPop: Paternal Cross: Type of cross (GEIT = geitonomous, SIB = Sibling, BW = Between populations, WIN = Within the same population, AUT = autogamous) TotSeeds: Total seeds produced from cross Date: Date cross was conducted   DATA-SPECIFIC INFORMATION FOR: GCWild_FloralMorphologyMaster.csv: 1. Number of variables: 18 2. Number of cases/rows: 604 3. Variable List: Species: G = O. gayleana, F = O. hartwegii subsp. filifolia Population: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Site: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Individual: individual plant ID number MatLine: Maternal line ID number Flower: flower measured, 1 = first, 2 = second, 3 = third, 4 - fourth Date: Date data were collected corolla_diameter_1: corolla diameter (mm) measurement perpendicular to corolla_diameter_2 (mm) corolla_diameter_2: corolla diameter (mm) measurement perpendicular to corolla_diameter_1 Floral_flare: diameter (mm) of the opening of the floral tube Filament_length1: length of first filament (mm) Filament_length2: length of second filament (mm) Style_length: length from floral flare to end of style (mm) Tube_length: length from floral flare to insertion at ovary (mm) H: Simplified negative, neutral, or positive herkogamy Notes: Notes taken during data collection Location: Location of plants included, GC = growth chamber, Wild = wild population sampled at study site   DATA-SPECIFIC INFORMATION FOR: GeneticAnalysis_Chloro.csv: 1. Number of variables: 10 2. Number of cases/rows: 19 3. Variable List: Sp: Species, F = O. hartwegii subsp. filifolia, G = O. gayleana Pop: Population, CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Scale: Landscape or local scale (Yeso hills) N: Number of individuals Na: Number of alleles Ne: Number of effective alleles I: Information index Hallo_Total: total number of haplotypes identified Unique: Number of haplotypes found only in that population   DATA-SPECIFIC INFORMATION FOR: GeneticAnalysis.csv: 1. Number of variables: 15 2. Number of cases/rows: 19 3. Variable List: Sp: Species, Ohf = O. hartwegii subsp. filifolia, G = O. gayleana Pop: Population, CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Scale: Landscape or local scale (Yeso hills) N = Number of individuals Na = Number of alleles Ne = Number of effective alleles I = Information index Ho = Observed heterozygosity He = Expected heterozygosity uHe = Unbiased expected heterozygosity Fis = Fixation index Prv = number of private alleles found in that population Avg = Average Relatedness   DATA-SPECIFIC INFORMATION FOR: Matrix_Distance.csv: 1. Number of variables: 8 2. Number of cases/rows: 9 3. Variable List: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Pairwise genetic distance given in km   DATA-SPECIFIC INFORMATION FOR: Matrix_FSTFili_Lin.csv: 1. Number of variables: 8 2. Number of cases/rows: 9 3. Variable List: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Pairwise FST values given   DATA-SPECIFIC INFORMATION FOR: Matrix_FstGay_Lin.csv: 1. Number of variables: 8 2. Number of cases/rows: 9 3. Variable List: CR = Croton, TR = Trigg Ranch, SH = Seven Hills, YA = Yeso A, YB = Yeso B, YC = Yeso C, YC = Yeso D, PHD = Paint Horse Draw Pairwise FST values given