# Title of Dataset: Raw data from: Natural alleles at the Doa locus underpin evolutionary changes in Drosophila lifespan and fecundity, by KM Hoedjes, H Kostic, L Keller, and T Flatt --- The dataset consists of an excel file with the raw data belonging to the figures of the main text and the supplementary material ## Description of the Data and file structure The data has been ordered in different tabs for each figure or supplementary file: "Fig2A+B": effect of RNAi of Doa on longevity GAL4 = the da-GS-GAL4 driver was used ("da") UAS = two UAS-RNAi constructs were used ("D10", "D19") or a control (w1118) RU = concentration of the drug (mifepristone) to induce RNAi (ug/ml) Cage = replicate cage number Sex = female (f) or male (m) Day_of_death = day of death of individual "Fig2C+D": effect of RNAi of Doa on fecundity (female egg laying rate) GAL4 = the da-GS-GAL4 driver was used ("da") UAS = two UAS-RNAi constructs were used ("D10", "D19") or a control (w1118) RU = concentration of the drug (mifepristone) to induce RNAi (ug/ml) Vial = replicate vial number Day = period of 3 days in which eggs were counted daily Average_egg_vial_day = average number of eggs per vial per day Average_egg_vial_female = average number of eggs per vial per day per female Sum_egg_vial = total number of eggs per vial during the experimental period "Fig 3": SNP association experiment to test the effect of four Doa SNPs on longevity SNP = name of the Doa SNP Allele = variant of the biallelic SNP F1_Cross_SNP = replicate F1 cross DGRP_Females = females of this DGRP line were used to generate the F1 cross DGRP_Males = males of this DGRP line were used to generate the F1 cross Cage = replicate cage number Sex = female (f) or male (m) Day_of_death = day of death of individual "Fig 4": SNP association experiment to test the effect of four Doa SNPs on fecundity SNP = name of the Doa SNP Allele = variant of the biallelic SNP F1_Cross_SNP = replicate F1 cross DGRP_Females = females of this DGRP line were used to generate the F1 cross DGRP_Males = males of this DGRP line were used to generate the F1 cross Vial = replicate vial number Day = day on which egg laying was assessed (3,6,9,12,15,18,21,24,27,30) Eggs_total = total number of eggs per vial per day Egg_female = average number of eggs per female per day "ESM2": quantitative real-time PCR experiment to confirm RNAi knockdown of Doa Sample = sample name Relative_expression = relative expression of the sample compared to the control treatment (0 RU) Gene = gene of which expression was measured = Doa Construct = RNAi construct of control RU = concentration of the drug (mifepristone) to induce RNAi (ug/ml) Batch = batch number (1 or 2) "ESM4": estimation of linkage disequilibrium (r2) between Doa SNPs Chr = chromosome (3R) pos1 = nucleotide position 1 pos2 = nucleotide position 2 rsq = r2 calculated between pos1 en pos2 "ESM6": allele frequency data of the four Doa SNPs and metadata of populations from the DEST database ID = population identifyer (from DEST) .._total = total sequence depth of each Doa SNP .._AF = allele frequency of the reference allele for each Doa SNP ## Sharing/access Information Data from the DEST database was used for a population genetic analysis (ESM6). This data is publicly available from: https://dest.bio/