# Title of Dataset: Normalised nanoluciferase values arising from use of positive reporter construct injected into Plutella xylostella eggs (moth luciferase raw data.1) or used in Aedes aegypti cell line (moth luciferase raw data.2). # Summary of experimental efforts underlying this dataset These data arise from the use of a positive reporter construct. This construct produces an mRNA coding for nanoluciferase whose translation is hindered by two hairpin structures in the UTRs. However, if the 3' hairpin is relased from the mRNA by cleavage (e.g. by a targeted piRNA or siRNA) then translation will increase. As such, levels of mRNa cleavage can be inferred from levels of nanoluciferase increase. # Description of file structure and contents moth luciferase raw data.1 Data are normalised luciferase values (nanoluciferase/Renilla luciferase). The positive reporter construct and a control renilla luciferase construct were injected into freshly laid p. xylostella eggs. Those eggs were sexed by PCR at 24hrs and normalised nanoluciferase calculated for each egg. Column 2 gives normalised values for eggs PCR's as female, Column 3 gives normalised values for eggs PCR'd as male. Column 4 gives values for non-injected controls (- control). moth luciferase raw data.2 Data are normalised luciferase values (nanoluciferase/Renilla luciferase). The positive reporter construct and a control renilla luciferase construct were tranfected into Aedes aegypti cells (Aag2 cells) alongside various concentrations of dsRNA matching either AmCyan (- control) or the PxyMAsc sequence targeted by Pxyfem and present in the positive reporter construct. Normalised luciferase values for each treatment represent the level of cutting of the construct mRNA in each treatment. Column 2&4 - cells transfected with AmCyan dsRNA. Columns 3&5 - cells transfected with PxyMasc mRNA. Column 6 - cells not transfected with dsRNA (Another - control) N/A represents unequal numbers of replicates, rather than missing data These are the luciferase readouts from both the preliminary experiments conducted in Aedes aegypti cell culture and then the secondary experiments conducted in Plutella xlostella embryos. The luciferase readouts give the activity of the positive readout system once targeted by the piRNA/siRNAs. ## Description of the Data and file structure All data is provided.The data consists of normalised luciferase data (nanoluc/renilla) for the above two experiments. Each experiment is described by a different worksheet (given above) and the titles of each experimental group are given as the top row in each column with the data for each replicate below it (as explained above). ## Sharing/access Information This is the only publicly accessible source for this information. Data is freely available. # Definitions of all variables, abbreviations, missing data codes, and units See above # Links to other publicly accessible locations of the data This is the only publicly accessible source for this information. # Other sources, if any, that the data was derived from N/A # Any other details that may influence reuse or replication efforts N/A