Files: Bliss_vp1_arcticborealbryo.tag.fasta Bliss_vp1_embl113.tag.fasta Bliss_vp2_arcticborealbryo.tag.fasta Bliss_vp2_embl113.tag.fasta Bliss_bryo_arcticborealbryo.tag.fasta Bliss_bryo_embl113.tag.fasta Bliss_diat_embl113.tag.fasta Bliss_cop_embl113.tag.fasta These files contain the unique sequences with their taxonomic identification inferred using the program ecotag (OBITools http://www.grenoble.prabi.fr/trac/OBITools). Sequences were produced by amplification of DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. DNA was amplified using the trnL-g and trnL-h primers to obtain sequences of the P6 loop of the tnrL, and amplicons were sequenced using Roche 454 GS FLX Titanium. All sequences presented are the output by the program ecotag are presented. Taxonomic identification was inferred using the reference libraries referred to in the file names ("arcticborealbryo", "embl113"). - "arcticborealbryo": a compilation of quality-checked and curated reference libraries for arctic and boreal species constructed at the Natural History Museum in Oslo (arctic vascular plants: Sønstebø et al. 2010; boreal vascular plants: Willerslev et al. 2014; bryophytes: Soininen et al. 2015). - "embl113": a reference library formatted from the EMBL Nucleotide Database standard release 113 by extracting sequences of the targeted region using ecoPCR (Ficetola et al. 2010). Details on sample collection, DNA extraction, PCR conditions, and tools and settings used for filtering and taxonomic identification of the raw sequence data, as well as further downstream analyses can be found in the associated publication. Names of the sequences in the fasta files begin with the sequence IDs used in Appendix_B.1-B.6 of the publication. Contact author: Laura S. Epp (laura.epp@awi.de) Sample information: Extraction name DNA sample depth(cm) Age(cal BP) EH1604 20 18-19 442 ± 139 EH1605 19 31-32 718 ± 162 EH1606 18 55-56 1228 ± 184 EH1607 17 70-71 1546 ± 189 EH1608 16 125-126 2714 ± 142 EH1609 15 157-158 4131 ± 240 EH1610 14 176-177 5485 ± 260 EH1611 13 198-199 6891 ± 121 EH1612 12 206-207 7119 ± 148 EH1613 11 215-217 7413 ± 155 EH1614 10 226-227 7754 ± 129 EH1615 9 234-236 8033 ± 116 EH1616 8 245-247 8397 ± 143 EH1617 7 253-255 8662 ± 133 EH1618 6 265-268 9176 ± 168 EH1619 5 275-277 9703 ± 228 EH1620 4 285-287 10257 ± 214 EH1621 3 289-291 10478 ± 188 EH1622 2 309-311 10676 ± 110 EH1623 1 330-331 10846 ± 105 References: Ficetola GF, Coissac E, Zundel S, Riaz T, Shehzad W, Bessiere J, Taberlet P, Pompanon F (2010) An In silico approach for the evaluation of DNA barcodes. Bmc Genomics 11, 434. Soininen EM, Gauthier G, Bilodeau F, Berteaux D, Gielly L, Taberlet P, Gussarova G, Bellemain E, Hassel K, Stenøien HK, Epp LS, Schrøder-Nielsen A, Brochmann C, Yoccoz NG (2015) Highly overlapping winter diet in two sympatric lemming species revealed by DNA metabarcoding Plos One 10, e0115335. Sønstebø JH, Gielly L, Brysting AK, Elven R, Edwards M, Haile J, Willerslev E, Coissac E, Rioux D, Sannier J, Taberlet P, Brochmann C (2010) Using next-generation sequencing for molecular reconstruction of past Arctic vegetation and climate. Molecular Ecology Resources 10, 1009-1018. Willerslev E, Davison J, Moora M, Zobel M, Coissac E, Edwards ME, Lorenzen ED, Vestergard M, Gussarova G, Haile J, Craine J, Gielly L, Boessenkool S, Epp LS, Pearman PB, Cheddadi R, Murray D, Brathen KA, Yoccoz N, Binney H, Cruaud C, Wincker P, Goslar T, Alsos IG, Bellemain E, Brysting AK, Elven R, Sonstebo JH, Murton J, Sher A, Rasmussen M, Ronn R, Mourier T, Cooper A, Austin J, Moller P, Froese D, Zazula G, Pompanon F, Rioux D, Niderkorn V, Tikhonov A, Savvinov G, Roberts RG, MacPhee RDE, Gilbert MTP, Kjaer KH, Orlando L, Brochmann C, Taberlet P (2014) Fifty thousand years of Arctic vegetation and megafaunal diet. Nature 506, 47-+.