### Description of file contents ## msap_raw_scores.csv Contains raw presence/absence scores of 129 MSAP bands across 234 individual grouse in 21 populations. This file is the input file for the R package "msap". Column 1: Population identifier Column 2: Individual identifier Column 3: Restriction enzyme ## msap_transformed_scores.txt Contains transformed presence/absence scores of 303 epiloci derived from the original 129 MSAP bands. Additionally, all relevant morphological data are included for regression tests. Column1: Individual identifier Column2: Population identifier Column3: Combarea in mm^2 Column4: Age (young or old) Column5: Weight in grams Column6: PCR-based sex Column7: Parasite load (worms) Columns ML1-ML303: Presence/absence of epiloci ## locus_ids.txt Contains epiloci names (ML1-ML303) with associated original MSAP band identifiers and methylation state (u=unmethylated, h=hemimethylated, m=fully methylated) Column1: Epilocus identifier Column2: MSAP band identifier Column3: Methylation state ## geo_distances.txt Contains a matrix with pairwise geographic distances among the 21 study populations. Use these distances for isolation-by-distance tests. ## R_analysis.r Contains the full R analysis of MSAP data (epigenetic structure, methylation levels, regression analyses).