ReadMe for "The Pillars of Hercules as a bathymetric barrier to gene-flow promoting isolation in a global deep-sea shark (Centroscymnus coelolepis)." 27-10-2015 This readme file describes the data files accompanying the above publication available at dryad. For any question please contact dianacatarino@uac.pt The following files are included: 1) "CC_microsatellites_genotypes.txt" This file contains the microsatellites genotypes for the 503 specimens of Centroscymnus coelolepis (CC). The file is in genepop 3 digits format, which is widely used and can be easily converted in other types of files. It contains the genotypes for the 11 nuclear loci screened in the 8 localities studied: AZ-Azores; SAZ-South of Azores; UKIRE-United Kingdom and Ireland; MAU-Mauritania; MED-Western Mediterranean; PT-Portugal mainland; MAD-Madeira; AUS-Australia. 2) "CC_CR_aligment_all.fas" This file contains all the 347 mtDNA Control Region sequences used in the above studied. The file is in fasta format with all the aligned sequences with 497bp.Localities codes: AZ-Azores; MAR-Mid-Atlantic Ridge; SAZ-South of Azores; UK-United Kingdom; IRE-Ireland; MAU-Mauritania; MED-Western Mediterranean; PT-Portugal mainland; MAD-Madeira; AUS-Australia; SAF-South Africa. 3) "CC_CR_aligment_haplotypes.fas" This file contains all 27 haplotypes found in the studied areas for Centroscymnus coelolepis. The file is in fasta format. Haplotypes frequencies: H1-121; H2-144; H3-3; H4-1: H5-20; H6-1; H7-1;H8-1; H9-1; H10-7; H11-1; H12-1; H13-6; H14-4; H15-2; H16-1; H17-1; H18-8; H19-10; H20-4; H21-1; H22-1; H23-1; H24-1; H25-1; H26-2; H27-2. 4) "CC_DB_biologic_data.xlsx" This file in excel format contains the biological data used in the life-history analyses. Each line refers to one sampled specimen and the columns refer to: Cruise/Source- Code for the scientific cruise where the samples were collected or in other case provenience of the samples; Data provider- Person responsible/owner for the collection of the biological data; Genetic code- Sample genetic code, specimens without a genetic code were not screened with genetic markers; Area- Geographic region of the specimens collected: Atlantic, Mediterranean, Australia; Date: specimen capture date; Depth: specimen capture depth in meters; Total length: Specimen total length in centimeters; Total weight: specimen total weight in kilograms; Sex: Male or female; Mature/immature; Source: data from this study or retrieved from the literature (only for some Australian samples). 5) "IMa_parameter_file.txt" This file contains the command lines with all the parameters used to run IMa2 software.IMa2 analyses input file was constructed from individual sequences and microsatellite genotypes for the subset of C. coelolepis samples. The subset consists of 53 samples drawn randomly from both Atlantic and Mediterranean groups (altogether 106 samples) and only samples with available microsatellite genotypes and mtDNA sequences were considered. The mutation rate was obtained from the DIYABC analysis.