The following files are relevant to the Plucain, Hindré, Le Gac, Tenaillon, Cruveiller, Médigue, Leiby, Harcombe, Marx, Lenski and Schneider 2014 paper: Frequency.Dependence.csv - the raw data for competitions with competitors at two initial ratios (1:9 and 9:1) to measure negative frequency-dependent interactions. Generic.Fitness.csv - the raw data for competitions with competitors at 1:1 initial ratios to measure fitness. Stable.Coexistence.csv - the raw data for competitions with competitors at two initial ratios (1:9 and 9:1) to measure stable coexistence over 11 days. Growth.in.L.Supernatant.csv - the raw data for 24-h growth in spent medium of the 6.5L4 clone. Metabolic.Flux.Analysis.csv - the raw data for estimates of metabolic fluxes. The data files have the following data: Frequency.Dependence.csv: Red strain provides the genotype of the competitor forming red colonies on indicator medium due to the inability to consume Arabinose (Ara- marker) or Maltose (Mal- marker) for ancestral or S genetic backgrounds, respectively. White strain provides the genotype of the competitor forming white colonies on indicator medium due to the ability to consume Arabinose (Ara+ marker) or Maltose (Mal+ marker) depending on the red strain competitor. Initial frequency of red strain is the volumetric frequency at the start of the competition of the competitor forming red colonies. Initial frequency of white strain is the volumetric frequency at the start of the competition of the competitor forming white colonies. T0 colony count of red strain is the count of red colonies on the plate with a 1/100 dilution factor at the start of the competition. T0 colony count of white strain is the count of white colonies on the plate with a 1/100 dilution factor at the start of the competition. T1 colony count of red strain is the count of red colonies on the plate with a 1/10,000 dilution factor after 1 day of the competition. T1 colony count of white strain is the count of white colonies on the plate with a 1/10,000 dilution factor after 1 day of the competition. Malthusian parameter for red strain is calculated as: ln(T1 colony count of red strain)*100 / ln(T0 colony count of red strain). Malthusian parameter for white strain is calculated as: ln(T1 colony count of white strain)*100 / ln(T0 colony count of white strain). Fitness of red strain relative to white strain is calculated as: Malthusian parameter for red strain / Malthusian parameter for white strain. Generic.Fitness.csv: Red strain provides the genotype of the competitor forming red colonies on indicator medium due to the inability to consume Arabinose (Ara- marker). White strain provides the genotype of the competitor forming white colonies on indicator medium due to the ability to consume Arabinose (Ara+ marker). T0 colony count of red strain is the count of red colonies on the plate with a 1/100 dilution factor at the start of the competition. T0 colony count of white strain is the count of white colonies on the plate with a 1/100 dilution factor at the start of the competition. T1 colony count of red strain is the count of red colonies on the plate with a 1/10,000 dilution factor after 1 day of the competition. T1 colony count of white strain is the count of white colonies on the plate with a 1/10,000 dilution factor after 1 day of the competition. Malthusian parameter for red strain is calculated as: ln(T1 colony count of red strain)*100 / ln(T0 colony count of red strain). Malthusian parameter for white strain is calculated as: ln(T1 colony count of white strain)*100 / ln(T0 colony count of white strain). Fitness of red strain relative to white strain is calcutated as: Malthusian parameter for red strain / Malthusian parameter for white strain. Stable.Coexistence.csv: Red strain provides the genotype of the competitor forming red colonies on indicator medium due to the inability to consume Arabinose (Ara- marker). White strain provides the genotype of the competitor forming white colonies on indicator medium due to the ability to consume Arabinose (Ara+ marker). Initial frequency of red strain is the volumetric frequency at the start of the competition of the competitor forming red colonies. Initial frequency of white strain is the volumetric frequency at the start of the competition of the competitor forming white colonies. D0 colony count of red strain is the count of red colonies on the plate with a 1/100 dilution factor at the start of the competition. D0 colony count of white strain is the count of white colonies on the plate with a 1/100 dilution factor at the start of the competition. D1 to D11 colony count of red strain is the count of red colonies on the plate with a 1/10,000 dilution factor at day 1 to 11 of the competition. D1 to D11 colony count of white strain is the count of white colonies on the plate with a 1/10,000 dilution factor at day 1 to 11 of the competition. D0 to D11 frequency of red strain is calculated as: colony count of red strain / (colony count of red strain + colony count of white strain) at day 1 to 11 of the competition. Growth.in.L.Supernatant.csv: Time refers to the time in hour of sampling of the cultures in L supernatant for each OD measurement. OD Anc strain refers to the OD values at 450nm for the growth of Anc strain in L supernatant over time, for each of the four replicates 1 to 4. OD Anc spoTevol arcAevol gntRevol strain refers to the OD values at 450nm for the growth of Anc spoTevol arcAevol gntRevol strain in L supernatant over time, for each of the four replicates 1 to 4. OD 6.5S1 strain refers to the OD values at 450nm for the growth of 6.5S1 strain in L supernatant over time, for each of the four replicates 1 to 4. Maximum OD Anc strain refers to the maximum OD value at 450nm for the growth of Anc strain in L supernatant for each of the four replicates 1 to 4. Maximum OD Anc spoTevol arcAevol gntRevol strain refers to the maximum OD value at 450nm for the growth of Anc spoTevol arcAevol gntRevol strain in L supernatant for each of the four replicates 1 to 4. Maximum OD 6.5S1 strain refers to the maximum OD value at 450nm for the growth of 6.5S1 strain in L supernatant for each of the four replicates 1 to 4. Maximum Growth Rate Anc strain refers to the maximum growth rate value calculated from the OD values at 450nm between 4.16h and 8.99h of growth of Anc strain in L supernatant, for each of the four replicates 1 to 4. Maximum Growth Rate Anc spoTevol arcAevol gntRevol strain refers to the maximum growth rate value calculated from the OD values at 450nm between 4.16h and 8.99h of growth of Anc spoTevol arcAevol gntRevol strain in L supernatant, for each of the four replicates 1 to 4. Maximum Growth Rate 6.5S1 strain refers to the maximum growth rate value calculated from the OD values at 450nm between 4.16h and 8.99h of growth of 6.5S1 strain in L supernatant, for each of the four replicates 1 to 4. Metabolic.Flux.Analysis.csv: Strain provides the genotype of the tested strain. Pathway refers to the metabolic pathway for which the flux is being estimated: the Entner-Doudoroff, glycolysis, and pentose phosphate pathways. Flux is the estimated proportion of metabolism that flows through that pathway. It is an average of 2-3 technical GC-MS replicate runs of a single biological sample. For the Entner-Doudoroff and glycolysis pathways, the values are point estimates. For the pentose phosphate pathway, the estimated values are upper bounds.