With the accompanying excel files, all analyses presented in Fricke, C; Avila-Calero, S and Armitage SAO (2020) Genotypes and their interaction effects on reproduction and mating-induced immune activation in Drosophila melanogaster can be reproduced. The main Excel file is organised by model numbers, which correspond to the models presented in table S2 in the Supporting Information 1. Data were obtained as described in the Material and Methods section of the article (please also refer to the Supporting Information 1 for more detailed explanations). Parameters used were: Female populations: Females were sourced from either the Dahomey (Dah) or Muenster (Ms) populations Male populations: Males were sourced from either the Dahomey (Dah) or Muenster (Ms) populations Latency: the time between introducing pairs to each other and the time they start mating in minutes Copulation duration: the time pairs spend in copula in minutes (some times shortened to cop_dur in table S2) ############# Models 1-4 and Models 12-15 ################################################### Egg number 6-24h: The number of eggs laid in the period from 6 to 24 hours after mating Offspring number 0-6h: The number of offspring produced from 0 to 6 hours after mating Offspring number 6-24h: The number of offspring produced from 6 to 24 hours after mating Offspring number 24-48h: The number of offspring produced from 24 to 48 hours after mating Egg-to-adult: the number of offspring eclosed from the eggs counted in the 6-24 period after mating Total offspring number: the number of offspring summed over all three intervals (0-48 hours after mating) [Interval in the model description in table S2 for model 2 and 13 refers to the number of offspring scored in one of the three intervals 0-6, 6-24 or 24-48 hours after mating] ############ Models 5-7 ################################################################### Freeze time: females were either snap frozen 3 hours or 6 hours after mating Offspring presence: we scored whether offspring were present (1) or absent (0) in the vials females had been housed in before being frozen ############# Model 9 and Model 10 ######################################################## Mated state: females were either mated once (m) before being frozen or time-matched virgins (v) Dipt: the expression level of Diptericin relative to the reference genes for pools of 7 females Dros: the expression level of Drosomycin relative to the reference genes for pools of 7 females Mtk: the expression level of Metchnikowin relative to the reference genes for pools of 7 females ############# Model 11 ##################################################################### male age: males were snap frozen either on the day of eclosion or 1, 3, 5 or 7 days after eclosion SP: the expression level of sex peptide (SP) for pools of 5 male abdomens ############ Model 12 - 15 ################################################################ Male genotype: females were either mated to males lacking and not transferring sex peptide (SP0) or sex-peptide transferring control males (SP+)