README This DRYAD package contains files that relate to: Miller et. al 2018. Anchored phylogenomics of Burrowing Mayflies (Ephemeroptera) and the evolution of tusks Please cite this publication, the DRYAD accession, and Genbank SRA accession number when using any of the data or the LEP1 enrichment kit. This DRYAD data package will includes 7 files, in addition to this README file: MAYFLY_ref.tar.gz: Folder containing the reference sequences by each loci used to make the Mayfly_1 probe kit as well as used as a set of references in IBA assembly. Mayfly_1_Probes.txt.gz: Mayfly_1 probe kit Miller_et_al_Alignments.tar.gz: Folder containing the alignments used for analyses. DN12_rDNA_ML.fas was used in IQ-TREE for the DNA1&2 ML analysis (code used is included at top of file). DN12_rDNA_MrBayes.nex was used in the DNA1&2+rDNA MrBayes analysis (code is at bottom of file). AA_for_ML.phy and rDNA_for_ML.phy were used in IQ-TREE for the AA+rDNA ML analysis (code is contained in the AA_rDNA_ML.nex file). AA_rDNA_Bayes.nex was used in the AA+rDNA MrBayes analysis (code included at the bottom). Miller_et_al_Loci.tar.gz: Folder contains alignments of each loci used in the study. Miller_et_al_ST1-ST2.xlsx: Supplementary tables for Miller_et_al 2017 that include genbank accessions. Miller_et_al_SF1.docx: Supplementary figures. Bayesian and Maximum Likelihood trees for AA+rDNA and DNA1&2+rDNA datasets. Miller_et_al_Trees.nwk: Trees in newick format, in the following order: AA+rDNA Bayesian, AA+rDNA ML, DNA1&2+rDNA Bayesian, DNA1&2+rDNA ML, Constraint.