Sewage loading and microbial risk in urban waters of the Great Lakes

McLellan, Sandra, University of Wisconsin-Milwaukee, https://orcid.org/0000-0003-3283-1151

Sauer, Elizabeth, University of Wisconsin-Milwaukee

Bootsman, Melinda, University of Wisconsin-Milwaukee

mclellan@uwm.edu, ep_sauer@yahoo.com, bootsma@uwm.edu

Published Mar 20, 2018 on Dryad. https://doi.org/10.15146/R3S962

Cite this dataset

McLellan, Sandra; Sauer, Elizabeth; Bootsman, Melinda (2018). Sewage loading and microbial risk in urban waters of the Great Lakes [Dataset]. Dryad. https://doi.org/10.15146/R3S962

Abstract

Theis dataset contains untreated sewage (wastewater treatment plant influent) samples from Milwaukee, WI, USA collected over a two year peroid. These were analyzed by qPCR for human associated indicators human Bacteroides (HB assay) and human Lachnospiraceae (Lachno2 assay), and by qPCR for E. coli and enterococci. Water samples were also collected at the estuary using a Telodyne ISCO sampler to perform automated, high frequnency sampling of 15 minute subsamples. Hourly samples were analyzed by culture for E. coli and enterococci. Composites of 1, 2, or 4 hours were analyzed by qPCR for These samples were analyzed by qCRP for HB, Lachno2, E. coli, and enterococci.

Usage notes

BLD is below the limit of detection

BLQ is below the limit of quantification, whihc is 225 CN/100 ml for all qPCR assays

Funding

National Institute of Allergy and Infectious Diseases, Award: R01AI091829

Milwaukee Metropolitan Sewerage District, Award: M03029P07