Skip to main content
Dryad logo

Rice-recombinant butyrylcholinesterase (rrBChE) production dataset

Citation

Liao, Shuting et al. (2021), Rice-recombinant butyrylcholinesterase (rrBChE) production dataset, Dryad, Dataset, https://doi.org/10.25338/B85W4H

Abstract

We propose a method for analyzing the variability in smooth, possibly nonlinear, functionals associated with a set of product production trajectories measured under different experimental conditions. The key challenge is to make meaningful inference on these parameters across different experimental conditions when only a limited number of measurements in time are collected for each treatment, and when there are only a few, or no, replicates available. For this purpose we adopt a modeling approach by representing the production trajectories in a B-spline basis, and develop a bootstrap-based inference procedure for the parameters of interest, also accounting for multiple comparisons. The methodology is applied to study two types of quantities of interest -- ''time to harvest'' and ''maximal productivity'' in the context of an experiment on the production of certain recombinant proteins under laboratory conditions. We complement the findings by extensive numerical experiments that look into the effects of different types of bootstrap procedures and associated schemes for computing p-values for tests of hypotheses.

Funding

National Aeronautics and Space Administration, Award: 19-19BRASH-2-0055

Defense Threat Reduction Agency, Award: #HDTRA1-15-0054

National Science Foundation, Award: DMS-1713120

National Science Foundation, Award: DMS-1915894

National Science Foundation, Award: DMS-1811405