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Dryad

Drosophila enhancer adult bristle expression assay using UAS sv-RNAi

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Aug 12, 2021 version files 42.21 KB

Abstract

Data from manuscript "Sex-specific evolution of a Drosophila sensory system via interacting cis- and trans-regulatory changes" by D Luecke, G Rice, and A Kopp.

This study examines the regulation of expression for the chemosensory bristle specifier gene Pox neuro, which has expanded expression into ancestrally mechanosensory bristles in male D. prolongata. Flourescent assays show qualitativetly expanded expression driven by the D. prolongata allele relative to enhancers from sister species in the developing pupal leg. Adult bristle disruption assays using shavenoid RNAi show quantitative expression expansion by the D. prolongata allele into specific mechanosensory bristles on the dorso-anterior leg of the first tarsi.