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Long-term Transverse Imaging of the Hippocampus with Glass Microperiscopes

Cite this dataset

Redman, William et al. (2022). Long-term Transverse Imaging of the Hippocampus with Glass Microperiscopes [Dataset]. Dryad.


The hippocampus consists of a stereotyped neuronal circuit repeated along the septal-temporal axis. This transverse circuit contains distinct subfields with stereotyped connectivity that support crucial cognitive processes, including episodic and spatial memory. However, comprehensive measurements across the transverse hippocampal circuit in vivo are intractable with existing techniques. Here, we developed an approach for two-photon imaging of the transverse hippocampal plane in awake mice via implanted glass microperiscopes, allowing optical access to the major hippocampal subfields and to the dendritic arbor of pyramidal neurons. Using this approach, we tracked dendritic morphological dynamics on CA1 apical dendrites and characterized spine turnover. We then used calcium imaging to quantify the prevalence of place and speed cells across subfields. Finally, we measured the anatomical distribution of spatial information, finding a non-uniform distribution of spatial selectivity along the DG-to-CA1 axis. This approach extends the existing toolbox for structural and functional measurements of hippocampal circuitry.

Usage notes

The data contains Microperiscope designs, Microsphere data from Figure 2, spine imaging data from Figure 3, and neuronal response data from Figures 5-6.

Code for point spread function calculations, spine analysis, and place/speed cell identification is available on Github (


National Science Foundation

National Institutes of Health

Whitehall Foundation

Larry L. Hillblom Foundation