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Data from: Ethanol abolishes vigilance-dependent astroglia network activation in mice by inhibiting norepinephrine release

Citation

Paukert, Martin et al. (2020), Data from: Ethanol abolishes vigilance-dependent astroglia network activation in mice by inhibiting norepinephrine release, Dryad, Dataset, https://doi.org/10.5061/dryad.08kprr516

Abstract

This collection of data sets was obtained during a study of the effect of acute ethanol intoxication on vigilance-dependent astroglia network activation in mice. The main findings have been that ethanol inhibits astroglia activation by inhibiting vigilance-dependent norepinephrine release. This leads to a failure of activation of alpha1A-adrenergic receptors on astroglia. Further, this study has revealed that ethanol inhibition of cerebellar Bergmann glia Ca2+ activation does not account for ataxic motor behavior, but may rather contribute to cognitive deficits.

Methods

The majority of data were acquired using two-photon Ca2+ imaging in awake behaving mice on a motorized linear treadmill, or in acute cerebellar slices. The data have been processed using MATLAB scripts. The study also includes quantification of motor coordination using the Catwalk XT system.

Usage Notes

All data are contained in one Excel file. The data of individual figures or supplementary figures have been organized into corresponding tabs. Within each tab the data are organized according to figure sub-panels. Annotations allow clear association of raw data with individual figure sub-panels (each dot within data plots is accounted for by its value in the Excel file). Statistical test results have been included with a mentioning of the type of test used.

Funding

National Institute on Alcohol Abuse and Alcoholism, Award: R01AA025128

National Institute of Mental Health, Award: R01MH113780

National Institute of Mental Health, Award: R01MH083728

The Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation

Deutsche Forschungsgemeinschaft, Award: A09N/SFB1158

Chica and Heinz Schaller Research Foundation