Within-female sperm allocation—Males inseminate at three different female body sites according to female mating history in a squid
Data files
Oct 10, 2023 version files 93.08 KB
-
Azad_master_dataset.xlsx
89.28 KB
-
README.md
3.80 KB
Abstract
Theory predicts that males should allocate their costly sperm according to future mate availability and focal female conditions, e.g. promiscuity and fecundity, to maximise lifetime reproductive success. Some squids show insemination site polymorphism within a female—males deposit sperm capsules at different female body locations. Whether the choice of insemination site is influenced by the sperm-storing status of the female remains untested. We studied the squid Loliolus sumatrensis – the females possess three insemination sites: the buccal membrane (BM), basal left IV arm (ARM) and lateral head behind the left eye (EYE). Analysis of seasonal dynamics identified a set priority for the initial use of insemination sites as BM then ARM then EYE, whereas the maximum number of stored spermatangia was greater as EYE>ARM>BM. The maturity status of females was correlated with the pattern, but not number, of inseminations. We found multiple paternity at all sites and the same genotypes in spermatangia collected from all three sites, suggesting male squids allocate their sperm to multiple insemination sites during a single mating episode according to the status of female sperm-storage. This study uncovered unusual within-female sperm allocation – male mating behaviours were influenced by female mating history.
README
Provenance for this README
- File name: README.MD
- Authors: Azad, Alam, Nagata, Tomano, Ono, Sugai and Hirohashi
- Date created: 2023-10-06
Dataset Version and Release History
- Current Version:
- Number: 1.0.0
- Date: 2023-10-06
- Persistent identifier: DOI: 10.5061/dryad.08kprr57x
- Summary of changes: n/a
Dataset Attribution and Usage
- Dataset Title: Data for the article "Within-female sperm allocation—Males inseminate at three different female body sites according to female mating history in a squid."
- Persistent identifier: DOI: 10.5061/dryad.08kprr57x
- Dataset contributors: Kamrun Naher Azad, Noritaka Hirohashi
- Dataset citation: <br> Kamrun Naher Azad, Md. Nur E-Alam, Makoto Nagata, Satoshi Tomano, Hiroki Ono, Kyoko Sugai, Noritaka Hirohashi (2022), Within-female sperm allocation—Males inseminate at three different female body sites according to female mating history in a squid. Dryad Digital Repository. (doi.10.5061/dryad.08kprr57x).
Contact Information
- Name: Noritaka Hirohashi
- Affiliations: Graduate School of Natural Science and Technology, Shimane University, Japan
- ORCID ID: https://orcid.org/0000-0001-9264-9517
- Email: hiro@life.shimane-u.ac.jp
- Address: e-mail preferred
Detailed Information of the Dataset
- Description of the dataset: This dataset contains squid individual somatic data obtained from 397 males and 520 females on eight different fishing days in 2021 and 2022.
Methods
The squid, Loliolus sumatrensis, were obtained from a fisherman at the Shodo Island, Kagawa, Japan during the fishery season (July-September) of this species in 2021 and 2022. the squid specimens were measured (mostly within one day after fishing) for dorsal mantle length (ML), body weight (BW), accessory gland weight (ACC), testis weight (TW), relative testis weight (RTW), ovary weight (OW), relative ovary weight (ROW), number of insemination sites used (SITE) and number of spermatangium attached to the female. To measure somatic weight, gonad weight (OW + ACC) was subtracted from total body weight.
Methods of data collection/generation: see manuscript for more details
File details
- This dataset consists of:
- The main analysis dataset, included directly in DataDryad as an Excel spreadsheet (Adad_master_dataset.xlsx) which contains the following worksheets:
- Somatic indices of adult male individuals in L. sumatrensis [sheet name; males] This file contains the data of somatic indices; mantle length, body weight, accessory reproductive gland weight, testis weight, relative testicular weight (TSI).
- Somatic indices of adult female individuals in L. sumatrensis [sheet name; females(+sperm_count) ] This file contains the data of somatic indices; mantle length, body weight, accessory reproductive gland weight, relative ovary weight (OSI), # of insemination sites used by males, # of spermatangia attached to each insemination site.BM: buccal membrane, ARM: left IV arm, EYE: behind the left eye.
- Somatic indices of adult female individuals in L. sumatrensis [sheet name; females(-sperm_count) ] This file contains the data of somatic indices; mantle length, body weight, accessory reproductive gland weight, relative ovary weight (OSI).
- Sperm size [sheet name; sperm]: This file contains the data of sperm flagellum length. Specimens collected from the male reproductive organ (spermatophoric sac) as well as the spermatangia attached to female body locations (BM, ARM and EYE).
- SSR development (sheet name; SSR): This file contains the data of fragment lengths of four SSR alleles determined by using genomic DNAs of 42 individuals.
Sharing/access Information
This dataset has not yet been deposited anywhere else.
END OF THE README