Data from: Fragility of foot process morphology in kidney podocytes arises from chaotic spatial propagation of cytoskeletal instability
Cite this dataset
Falkenberg, Cibele V. et al. (2018). Data from: Fragility of foot process morphology in kidney podocytes arises from chaotic spatial propagation of cytoskeletal instability [Dataset]. Dryad. https://doi.org/10.5061/dryad.09d0k
The kidney podocyte's function depends on finger-like projections (foot processes) that interdigitate with those from neighboring cells to form the glomerular filtration barrier. The integrity of the barrier depends on spatial control of dynamics of actin cytoskeleton in the foot processes. We determined how imbalances in regulation of actin cytoskeletal dynamics can result in pathological morphology. We obtained 3-D electron microscopy images of podocytes and used quantitative features to build dynamical models to investigate how regulation of actin dynamics within foot processes controls local morphology. We find that imbalances in regulation of actin bundling, lead to chaotic spatial patterns that could impair the foot process morphology. Simulation results are consistent with experimental observations for cytoskeletal reconfiguration by dysregulated RhoA or Rac1, and they predict compensatory mechanisms for biochemical stability. We conclude that podocyte morphology, optimized for filtration, is intrinsically fragile, whereby local transient biochemical imbalances may lead to permanent morphological changes associated with pathophysiology.